Background: Bougainvillea x buttiana has long been used as a Mexican conventional medicinal drug to cure different sicknesses.

Objective: In this work, the consequences of ethanol combination and temperature extraction for phenolic compound recovery with the biological activity in Bougainvillea x buttiana extracts were investigated.

Materials and Methods: The phenolic recovery from Bougainvillea x buttiana was determined by comparing the effects of ethanol 0%, 50-100% (v/v) and extraction temperature (26, 45 and 64°C). The total phenolic content was investigated using spectrophotometric methods. Antioxidant activity from different Bougainvillea x buttiana extracts was evaluated by determining their potential to scavenge the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical. The chemical compositions and cytotoxic effect of extracts were determined using GC-MS techniques and the crystal violet technique, respectively.

Results: Our results confirmed that all extractions with different concentrations and temperatures had significant effects over the total phenolic contents and antioxidant activity (DPPH) of the extracts. The best recuperation of the total phenol content was observed in extracts with 60% ethanol at 26°C (201.08 mgEGA/g dry extract). Extracts with 100% ethanol at 26°C showed the highest antioxidant activity (IC50 was 286.75 μg/mL). GC-MS revealed the presence of various phytochemical contents with polar and non-polar properties. The phenolic compounds were investigated as to the structure, quantity and position of the hydroxyl groups on the benzene ring.

Conclusion: The ethanolic extract of Bougainvillea x buttiana was confirmed to have high phenolic content and contain pharmacologically active compounds, with potent antioxidant effects and low cytotoxic effects.

背景：九重葛x丁香已长期用作墨西哥传统的药物来治疗不同的疾病。

目的：在这项工作中，研究了乙醇组合和温度提取对九重葛提取物中具有生物活性的酚类化合物回收的影响。

材料和方法：通过比较0％，50-100％（v / v）乙醇和提取温度（26、45和64°C）的影响，确定从九重葛中提取的酚。使用分光光度法研究了总酚含量。通过确定其清除2，2-二苯基-1-吡啶并肼基（DPPH）自由基的潜力，评估了来自不同的九重葛提取物的抗氧化活性。分别使用GC-MS技术和结晶紫技术确定提取物的化学成分和细胞毒性作用。

结果：我们的结果证实，所有不同浓度和温度的提取物对提取物的总酚含量和抗氧化活性（DPPH）都有显着影响。在26°C的条件下，用60％乙醇提取的总苯酚含量最佳回收（201.08 mgEGA / g干提取物）。在26°C下用100％乙醇提取的化合物具有最高的抗氧化活性（IC50为286.75μg/ mL）。GC-MS揭示了具有极性和非极性特性的各种植物化学成分的存在。研究了酚类化合物在苯环上羟基的结构，数量和位置。

结论：九重葛的乙醇提取物被证实具有较高的酚含量，并含有药理活性化合物，具有强大的抗氧化作用和低细胞毒性作用。