Introduction. This study aimed to evaluate the long-term impact of new calcium silicate-based sealer (HiFlow Bioceramic) compared to calcium hydroxide-based sealer (Sealapex) on mineral constituents and crystallinity of radicular dentin. Methods. Roots of multirooted maxillary molars were denuded their dentin from all covered cementum then longitudinally split. The dentin segments were divided into three groups covered with either HiFlow root canal sealer or Sealapex root canal sealer or kept untreated (control group). After complete setting of sealers, the radicular dentin of each group was stored in phosphate buffer solution or deionized water for 60 days and then examined with Fourier Infrared Spectroscopy, scanning electron microscope/energy dispersed X-ray and X-ray diffraction (XRD). Results. When the dentin was stored in phosphate buffer solution, compared with untreated dentin, Fourier transform infrared spectra showed insignificant increase in the amide I and phosphate, significant increase in carbonate area, and carbonate/phosphate ratio, while there was insignificant change in phosphate/amide I ratio of HiFlow-treated dentin, whereas Sealapex induced significant decrease in amide I area, phosphate area, and phosphate/amide ratio. Both sealers induced significant increase in crystallinity index and significant decrease in crystallinity percent. Energy dispersed X-ray showed decrease in calcium content by both sealers. The phosphate content was increased by HiFlow and decreased by Sealapex. Conclusions. The mineral composition and crystallinity of dentin were insignificantly changed by the long-term HiFlow treatment, whereas, markedly changes by Sealapex.

中文翻译：

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新型基于硅酸钙的密封剂对根部牙本质矿物质含量和结晶度的长期影响：一项体外研究

引言。这项研究旨在评估与基于氢氧化钙的密封剂（Sealapex）相比，新型基于硅酸钙的密封剂（HiFlow Bioceramic）对放射性牙本质的矿物质成分和结晶度的长期影响。方法。从所有覆盖的牙骨质上剥除多根上颌磨牙的牙本质，然后纵向裂开。将牙本质段分为三组，分别用HiFlow根管封闭剂或Sealapex根管封闭剂覆盖或不进行治疗（对照组）。密封剂完全固化后，将每组的放射状牙本质储存在磷酸盐缓冲溶液或去离子水中60天，然后用傅立叶红外光谱仪，扫描电子显微镜/能量分散X射线和X射线衍射（XRD）进行检查。结果。当牙本质储存在磷酸盐缓冲溶液中时，与未处理的牙本质相比，傅立叶变换红外光谱显示酰胺I和磷酸盐的增加不明显，碳酸盐面积和碳酸盐/磷酸盐的比率显着增加，而磷酸盐/酰胺的变化不明显用HiFlow处理的牙本质的I比率，而Sealapex导致酰胺I面积，磷酸盐面积和磷酸盐/酰胺比率显着降低。两种密封剂均导致结晶度指数显着增加和结晶度百分比显着下降。能量分散的X射线显示两种密封剂的钙含量均降低。磷酸含量通过HiFlow增加，而Sealapex降低。结论。长期的HiFlow处理对牙本质的矿物质组成和结晶度影响不大，而Sealapex对牙本质的矿物质组成和结晶度影响不大。