Abstract In this study, a new lactose biosensor has been developed in which the 3,4-ethylenedioxythiophene/thiophene (EDOT/Th) copolymer is used as a transducer. The EDOT/Th copolymer was deposited on the glassy carbon electrode to be used as the working electrode. In addition to the working electrode, the three-electrode system was used in both the electrochemical synthesis and in the biosensor measurements. Lactase (β-galactosidase) that catalyzes the breakdown of lactose into monosaccharides (glucose and galactose) and galactose oxidase that catalyzes the oxidation of the resulting galactose were attached to the copolymer by a cross-linker on the modified working electrode. The response of the enzyme electrode to lactose was determined by cyclic voltammetry (CV) at +0.12 V. Enzyme electrode optimization parameters (pH, temperature, enzyme concentration, etc.) were performed. Fourier transform infrared spectroscopy, scanning electron microscopy and CV methods were used to support copolymer formation. In addition, the characteristics of the enzyme electrode prepared in this study (K m, 0.02 mM; activation energy E a, 38 kJ/mol; linear working range, up to 1.72 mM; limit of detection, 1.9 × 10−5 M and effects of interferents [uric acid and ascorbic acid]) were determined.

中文翻译：

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基于电化学合成的 3,4-乙撑二氧噻吩/噻吩 (EDOT/Th) 共聚物的新型乳糖生物传感器

摘要 在这项研究中，开发了一种新的乳糖生物传感器，其中 3,4-亚乙基二氧噻吩/噻吩 (EDOT/Th) 共聚物用作传感器。EDOT/Th 共聚物沉积在玻璃碳电极上用作工作电极。除了工作电极外，三电极系统还用于电化学合成和生物传感器测量。催化乳糖分解成单糖（葡萄糖和半乳糖）的乳糖酶（β-半乳糖苷酶）和催化所得半乳糖氧化的半乳糖氧化酶通过修饰工作电极上的交联剂连接到共聚物上。酶电极对乳糖的响应通过循环伏安法 (CV) 在 +0.12 V 下确定。酶电极优化参数（pH、温度、酶浓度等）。傅里叶变换红外光谱、扫描电子显微镜和 CV 方法用于支持共聚物的形成。此外，本研究制备的酶电极的特性（K m，0.02 mM；活化能 E a，38 kJ/mol；线性工作范围，高达 1.72 mM；检测限，1.9 × 10−5 M 和干扰物 [尿酸和抗坏血酸]）的影响进行了测定。