Infectious diseases and aetiological agents related to female reproductive systems were extensively covered compared to its male counterpart. There needs a proper study to bridge this gap, where microflora and infectious agents of both male and female reproductive are mutually intelligible. With this study, we aimed to evaluate the microbial contamination of the preputial cavity and also screened for abortion‐causing agents which are zoonotic as well. In goats, such types of abortions are caused by Brucella melitensis, Chlamydophila, Campylobacter and Coxiella etc. One of the major sources of contamination of semen is the preputial cavity, which is exposed to the external environment leading to spread of infection into the female via semen straws or by natural service. In the current study, good quality bucks (n = 32, Barbari = 12, Jamunapari = 10, Jakhrana = 10) which were routinely used for semen collection were screened for their preputial swabs, for the presence of the above pathogens. For detection of Brucella melitensis, OMP31 based TaqMan® probe real‐time PCR assay was used, and for Chlamydia, 16srRNA gene based SYBR® green real‐time PCR assay was employed for detection of Chlamydophila abortus. While for Campylobacter spp. and Coxiella burnetii, 16srRNA gene based conventional PCR and Trans‐PCR were used, respectively. In the current study, of the screened preputial swabs, none of them showed positive for Brucella and Coxiella, but of the screened 32 samples 17 showed positive for Chlamydia (53.13%) and two (6.25%) showed positive for Campylobacter spp. The current study emphasizes on the farms and laboratories which were regularly involved in screening of brucellosis also often overlook the other potential non‐brucella pathogens, causing abortions eventually incurring severe economic losses to the goat keepers.

中文翻译：

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使用基于PCR的检测方法对种公羊前牙拭子中重要的引起堕胎的微生物进行分子检测。

与男性生殖系统相比，与女性生殖系统有关的传染病和病原体得到了广泛报道。需要进行适当的研究来弥合这种差距，在这种差距中，微生物菌群和雄性和雌性生殖的传染原是相互可理解的。通过这项研究，我们旨在评估前牙腔的微生物污染，并筛选出人畜共患的人工流产药物。在山羊中，此类流产是由布鲁氏菌，衣原体，弯曲杆菌和Coxiella等引起的。。精液污染的主要来源之一是包皮腔，该包皮腔暴露于外部环境，导致感染通过精液吸管或自然服务传播给女性。在当前的研究中， 筛选了常规用于收集精液的优质雄鹿（n = 32，Barbari = 12，Jamunapari = 10，Jakhrana = 10），以检查是否存在上述病原体，并用其拭子拭子。为了检测布鲁氏菌，使用基于OMP31的TaqMan®探针实时PCR分析法，对于衣原体，使用基于16srRNA基因的SYBR®绿色实时PCR分析法检测流产衣原体。而对于弯曲杆菌属。和柯氏杆菌，分别使用了基于16srRNA基因的常规PCR和Trans-PCR。在当前的研究中，在筛查的包皮拭子中，没有一个对布鲁氏菌和Coxiella呈阳性，但在所筛查的32个样品中，有17个对衣原体呈阳性（53.13％），两个（6.25％）对弯曲杆菌属呈阳性。当前的研究强调了经常参与布鲁氏菌病筛查的农场和实验室，也常常忽视了其他潜在的非布鲁氏菌病原体，导致流产，最终给养山羊者造成严重的经济损失。