Dermatophytoses are infections that affect keratinized tissues. Their main etiologic agents are fungi of the genera Microsporum and Trichophyton. The emergence of resistant fungi and the clinical relevance of dermatophytosis has encouraged studies that aim to increase the arsenal of drugs or act on mechanisms that confer multiple drug resistance. This study investigated the modulating activity of terbinafine promoted by dihydrojasmone and terpinolene against Microsporum canis LM 216, Trichophyton interdigitale H6 and Trichophyton interdigitaleΔmdr2. The minimum inhibitory concentration (MIC) of test drugs was determined by broth microdilution. The effect of the drugs tested on plasma membrane functionality was analyzed. Terbinafine MIC was determined in subinhibitory concentrations of monoterpenes. Finally, it was performed an association study with terbinafine and monoterpenes. Dihydrojasmone presented lower MIC values than terpinolene. All fungi were sensitive to terbinafine, starting at 1 μg mL-1 . All tested drugs increased K+ release (p <0.05), affecting the functionality of the plasma membrane. Dihydrojasmone modulated the sensitivity of all strains against terbinafine, and terpinolene modulated the sensitivity of M. canis LM 216 and T. interdigitale Δmdr2. The monoterpenes and terbinafine drug associations presented synergism. In conclusion, the results suggest that the dihydrojasmone and terpinolene are promising antifungal agents that potentiates the antifungal activity of terbinafine against dermatophytes.

中文翻译：

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二氢茉莉酮和萜品油烯增强特比萘芬对皮肤癣菌的抗真菌活性

皮肤癣菌病是影响角化组织的感染。它们的主要病原体是小孢子菌属和毛癣菌属的真菌。耐药真菌的出现和皮肤癣菌病的临床相关性促进了旨在增加药物库或作用于赋予多重耐药性的机制的研究。本研究调查了由二氢茉莉酮和萜品油烯促进的特比萘芬对犬小孢子菌 LM 216、叉指毛癣菌 H6 和叉指毛癣菌Δmdr2 的调节活性。受试药物的最低抑菌浓度 (MIC) 由肉汤微量稀释法确定。分析了测试的药物对质膜功能的影响。特比萘芬 MIC 在单萜的亚抑制浓度下测定。最后，对特比萘芬和单萜进行了关联研究。二氢茉莉酮的 MIC 值低于萜品油烯。所有真菌对特比萘芬敏感，从 1 μg mL-1 开始。所有测试的药物都会增加 K+ 释放（p <0.05），影响质膜的功能。二氢茉莉酮调节所有菌株对特比萘芬的敏感性，萜品油烯调节 M. canis LM 216 和 T. interdigitale Δmdr2 的敏感性。单萜和特比萘芬药物组合表现出协同作用。总之，结果表明二氢茉莉酮和萜品油烯是有前景的抗真菌剂，可增强特比萘芬对皮肤癣菌的抗真菌活性。从 1 μg mL-1 开始。所有测试的药物都会增加 K+ 释放（p <0.05），影响质膜的功能。二氢茉莉酮调节所有菌株对特比萘芬的敏感性，萜品油烯调节 M. canis LM 216 和 T. interdigitale Δmdr2 的敏感性。单萜和特比萘芬药物组合表现出协同作用。总之，结果表明二氢茉莉酮和萜品油烯是有前景的抗真菌剂，可增强特比萘芬对皮肤癣菌的抗真菌活性。从 1 μg mL-1 开始。所有测试的药物都会增加 K+ 释放（p <0.05），影响质膜的功能。二氢茉莉酮调节所有菌株对特比萘芬的敏感性，萜品油烯调节 M. canis LM 216 和 T. interdigitale Δmdr2 的敏感性。单萜和特比萘芬药物组合表现出协同作用。总之，结果表明二氢茉莉酮和萜品油烯是有前景的抗真菌剂，可增强特比萘芬对皮肤癣菌的抗真菌活性。单萜和特比萘芬药物组合表现出协同作用。总之，结果表明二氢茉莉酮和萜品油烯是有前景的抗真菌剂，可增强特比萘芬对皮肤癣菌的抗真菌活性。单萜和特比萘芬药物组合表现出协同作用。总之，结果表明二氢茉莉酮和萜品油烯是有前景的抗真菌剂，可增强特比萘芬对皮肤癣菌的抗真菌活性。