Salmonella enterica, Staphylococcus aureus, Escherichia coli O157: H7, and Listeria monocytogenes may contaminate similar types of food and cause foodborne disease. The objective of this study was to develop a selective enrichment broth for simultaneous enrichment of Salmonella enterica, Staphylococcus aureus, Escherichia coli O157: H7, and Listeria monocytogenes (SSEL) using nalidixic acid, acriflavine, lithium chloride, and sodium cholate as selective agents. Developed SSEL broth not only enriched the target pathogens to 5 log10 CFU/ml after 18 hr incubation at 37°C with 10–100 CFU/mL of inoculation concentration, but also could successfully support the simultaneous enrichment of target pathogens with similar growth rates and inhibit the growth of most nontarget bacteria effectively. The enrichment effect of SSEL was confirmed by artificial contamination test coupled with multiplex PCR. In summary, SSEL has been shown to be a promising multiplex selective enrichment broth for the detection of the four pathogens on a single‐assay platform.

中文翻译：

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SSEL，用于同时生长肠沙门氏菌，金黄色葡萄球菌，大肠杆菌O157：H7和单核细胞增生李斯特菌的选择性富集肉汤

肠炎沙门氏菌，金黄色葡萄球菌，大肠杆菌O157：H7和单核细胞增生性李斯特菌可能污染相似类型的食物并引起食源性疾病。这项研究的目的是开发一种选择性富集肉汤，以同时富集肠沙门氏菌，金黄色葡萄球菌，大肠杆菌O157：H7和单核细胞增生李斯特菌（SSEL），使用萘啶酸，a啶黄，氯化锂和胆酸钠作为选择剂。发达的SSEL肉汤不仅可以在37°C下以10–100 CFU / mL的接种浓度将目标病原体富集至5 log10 CFU / ml，而且还可以成功地支持以相似的生长速率和同时富集目标病原体。有效抑制大多数非目标细菌的生长。通过人工污染试验结合多重PCR证实了SSEL的富集效果。总而言之，SSEL已被证明是一种有前途的多重选择性富集肉汤，可用于在单一测定平台上检测四种病原体。