Uric acid is a potential metabolite that serves as a danger‐associated molecular pattern (DAMP) and induces inflammatory responses in sterile environments. Porphyromonas gingivalis is a keystone periodontopathogen, and its gingipain proteases play a critical role in the pathogenesis of periodontitis. In this study, we demonstrate that P. gingivalis gingipains play a role in THP‐1 macrophage uric acid production by increasing the expression and activity of xanthine oxidoreductase (XOR). Uric acid sodium salt induces caspase‐1 activation, cell death, and the expression of proinflammatory cytokines, including IL‐1α, IL‐6, and IL‐8, in the human keratinocyte HOK‐16B cell line. Our results suggest that gingipain‐induced uric acid can mediate inflammation in periodontal tissue cells.

中文翻译：

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牙龈卟啉单胞菌牙龈蛋白酶产生的尿酸的炎症反应。

尿酸是一种潜在的代谢产物，可作为危险相关分子模式（DAMP），并在无菌环境中引起炎症反应。牙龈卟啉单胞菌是牙周病的主要病因，其牙龈蛋白酶在牙周炎的发病过程中起着至关重要的作用。在这项研究中，我们证明牙龈卟啉单胞菌牙龈蛋白酶通过增加黄嘌呤氧化还原酶（XOR）的表达和活性在THP-1巨噬细胞尿酸生产中发挥作用。尿酸钠在人角质形成细胞HOK-16B细胞系中诱导caspase-1活化，细胞死亡和促炎细胞因子的表达，包括IL-1α，IL-6和IL-8。我们的结果表明，姜黄素诱导的尿酸可以介导牙周组织细胞的炎症。