The Hippo pathway is a tumor suppressive pathway regulating Yes‐associated protein‐TEA domain‐containing sequence‐specific transcription factor (YAP‐TEAD) complex. VGLL (Vestigial‐like) proteins are transcriptional cofactors competing with YAP for TEAD binding and interfering oncogenic activity of YAP‐TEAD complex. We evaluated the expression of VGLL4, YAP, and TEAD4 and assessed their correlations with clinicopathologic factors and prognostic effects in 295 colorectal cancers. VGLL4 was positive in 164 (55.6%) cases and correlated with small tumor size, low pT classification, and absence of lymph node metastasis. YAP and TEAD4 were highly expressed in 138 (46.8%) cases and 144 (48.8%) cases, respectively, and high expressions were associated with presence of lymphovascular invasion and lymph node metastasis, or distant metastasis. VGLL4 expression was significantly correlated with low YAP expression (p < 0.001) and had significantly better overall survival than negative expression (p < 0.001). High YAP (HR, 2.108; 95% confidence interval, 1.239–3.584; p = 0.006) and TEAD4 (1.724; 1.021–2.912; p = 0.042) expressions were associated with poor overall survivals. The combined VGLL4^posYAP^low expression showed the best overall survival than other groups (p < 0.001). VGLL4 expression (0.381; 0.212–0.683; p = 0.001) and combined VGLL4^posYAP^low expression (0.227; 0.108–0.475; p < 0.001) were independent good prognostic factors in colorectal cancers. The expressions of VGLL4, YAP, and TEAD4 can be used as prognostic markers in colorectal cancer patients.

中文翻译：

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YAP表达低的VGLL4与结直肠癌预后良好相关。

Hippo途径是一种肿瘤抑制途径，调节含有Yes相关蛋白-TEA结构域的序列特异性转录因子（YAP-TEAD）复合体。VGLL（Vestigial-like）蛋白是与YAP竞争TEAD结合并干扰YAP-TEAD复合物致癌活性的转录辅因子。我们评估了VGLL4，YAP和TEAD4的表达，并评估了它们与295例结直肠癌的临床病理因素和预后的相关性。VGLL4阳性164例（55.6％），与肿瘤小，pT分类低和无淋巴结转移有关。YAP和TEAD4分别在138（46.8％）和144（48.8％）例中高表达，并且高表达与淋巴管浸润和淋巴结转移或远处转移相关。VGLL4表达与低YAP表达显着相关（p <0.001），并且比阴性表达具有更好的总体存活率（p <0.001）。高的YAP（HR，2.108； 95％置信区间，1.239–3.584； p = 0.006）和TEAD4（1.724； 1.021–2.912； p = 0.042）表达与总体生存期差有关。组合的VGLL4^pos YAP^低表达显示出比其他组最佳的总体生存（p <0.001）。VGLL4表达（0.381; 0.212–0.683; p = 0.001）和VGLL4 ^pos YAP联合^低表达（0.227; 0.108–0.475; p <0.001）是结直肠癌的独立的良好预后因素。VGLL4，YAP和TEAD4的表达可用作结直肠癌患者的预后标志物。