Despite the Cox inhibitory anti-inflammatory and antipyretic effects of most widely used non-steroidal anti-inflammatory drugs (NSAIDs), such as Ibuprofen, their chronic use is associated with a plethora of patho-physiological insults. One such toxic effect on testicular tissues is not well studied and the underlying molecular mechanisms remain unexplored. Thus, the current study is designed to evaluate the antioxidant properties of essential trace element selenium (Se) to ameliorative Ibuprofen associated testicular toxic effects. Adult male Wistar rats were divided into 3 groups and fed on diets containing different concentrations of sodium selenite, viz. 0.01 mg/kg (Se- deficient), 0.2 mg/kg (Se-adequate), or 0.5 mg/kg (Se- supplemented) for 8 weeks. After diet feeding schedule, each group was divided into two subgroups i.e., with or without the treatment of Ibuprofen (120 mg/kg Bw). The protective effect of Se was evaluated by measuring testicular Se and selenoproteins status, spermatogenic markers, histopathology and testicular redox status. Ibuprofen diminished seminal volume, sperm count, sperm motility, which correlated well increased testicular reactive oxygen species. Se deficiency exacerbated these detrimental effects of ibuprofen by increasing oxidative stress. Alternatively, Se supplementation through antioxidant enzymes mediated protective effects. Se as essential antioxidant selenoproteins ameliorates Ibuprofen induced male reproductive toxicity.

尽管最广泛使用的非甾体抗炎药 (NSAID) 如布洛芬具有 Cox 抑制性抗炎和解热作用，但它们的长期使用与过多的病理生理损伤有关。其中一种对睾丸组织的毒性作用尚未得到充分研究，其潜在的分子机制仍未探索。因此，目前的研究旨在评估必需微量元素硒 (Se) 对改善布洛芬相关睾丸毒性作用的抗氧化特性。成年雄性 Wistar 大鼠分为 3 组，喂食含有不同浓度亚硒酸钠的饮食，即。0.01 mg/kg（缺硒）、0.2 mg/kg（充足硒）或 0.5 mg/kg（补硒），持续 8 周。饮食喂养计划后，每组分为两个亚组，即 有或没有布洛芬 (120 mg/kg Bw) 的治疗。通过测量睾丸硒和硒蛋白状态、生精标志物、组织病理学和睾丸氧化还原状态来评估硒的保护作用。布洛芬减少了精液量、精子数量、精子活力，这与睾丸活性氧物质的增加密切相关。硒缺乏通过增加氧化应激加剧了布洛芬的这些不利影响。或者，通过抗氧化酶补充硒介导了保护作用。硒作为必需的抗氧化剂硒蛋白可改善布洛芬诱导的男性生殖毒性。布洛芬减少了精液量、精子数量、精子活力，这与睾丸活性氧物质的增加密切相关。硒缺乏通过增加氧化应激加剧了布洛芬的这些不利影响。或者，通过抗氧化酶补充硒介导了保护作用。硒作为必需的抗氧化剂硒蛋白可改善布洛芬诱导的男性生殖毒性。布洛芬减少了精液量、精子数量、精子活力，这与睾丸活性氧物质的增加密切相关。硒缺乏通过增加氧化应激加剧了布洛芬的这些不利影响。或者，通过抗氧化酶补充硒介导了保护作用。硒作为必需的抗氧化剂硒蛋白可改善布洛芬诱导的男性生殖毒性。