Abstract Lanthanide complex-based luminescent probes have shown great utilities in detecting various analytes by time-gated luminescence imaging technology. However, few lanthanide probes are suitable for the monitoring of bioactive species in organelles. Furthermore, the common UV excitation windows of most lanthanide probes limit their application in living biological samples. In this work, a novel visible-light-excited europium(III) complex-based luminescent probe, [Eu(Lyso-CDHH)3(DPBT)], was designed and synthesized for the detection of exogenous and endogenous hypochlorous acid (HOCl) in lysosomes. The probe exhibits a wide excitation window from ultraviolet to visible region with a maximum excitation wavelength at 400 nm and a high luminescence quantum yield (41.7%). Upon reaction with HOCl, the luminescence of the probe can be quenched within 5 s and displays strong resistance to the interferences from other reactive species. The designed probe also shows high sensitivity towards HOCl with a low detection limit at micromolar concentration level. In addition, the good cell-membrane permeability and the lysosome-targetability of the probe enabled its successful application in the time-gated luminescence imaging of exogenous and endogenous HOCl in lysosomes of RAW 264.7 cells. This work will shed lights on the design of visible-light-excited and organelle-targetable lanthanide molecular probes for broader time-gated luminescence application.

中文翻译：

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开发一种可溶酶体靶向的可见光激发铕 (III) 复合物发光探针，用于对活细胞中的次氯酸成像

摘要 基于镧系元素配合物的发光探针在通过时间门控发光成像技术检测各种分析物方面显示出巨大的效用。然而，很少有镧系元素探针适合监测细胞器中的生物活性物质。此外，大多数镧系元素探针的常见紫外激发窗口限制了它们在活生物样品中的应用。在这项工作中，设计并合成了一种新型的基于可见光激发的铕 (III) 配合物的发光探针 [Eu(Lyso-CDHH)3(DPBT)]，用于检测外源性和内源性次氯酸 (HOCl)在溶酶体中。该探针具有从紫外区到可见区的宽激发窗口，最大激发波长为 400 nm，发光量子产率高（41.7%）。与 HOCl 反应后，探针的发光可在 5 s 内淬灭，对其他活性物质的干扰具有很强的抵抗力。设计的探针还显示出对 HOCl 的高灵敏度，在微摩尔浓度水平上具有低检测限。此外，探针良好的细胞膜渗透性和溶酶体靶向性使其成功应用于 RAW 264.7 细胞溶酶体中外源性和内源性 HOCl 的时间门控发光成像。这项工作将阐明可见光激发和细胞器靶向镧系元素分子探针的设计，以用于更广泛的时间门控发光应用。设计的探针还显示出对 HOCl 的高灵敏度，在微摩尔浓度水平上具有低检测限。此外，探针良好的细胞膜渗透性和溶酶体靶向性使其成功应用于 RAW 264.7 细胞溶酶体中外源性和内源性 HOCl 的时间门控发光成像。这项工作将阐明可见光激发和细胞器靶向镧系元素分子探针的设计，以用于更广泛的时间门控发光应用。设计的探针还显示出对 HOCl 的高灵敏度，在微摩尔浓度水平上具有低检测限。此外，探针良好的细胞膜渗透性和溶酶体靶向性使其成功应用于 RAW 264.7 细胞溶酶体中外源性和内源性 HOCl 的时间门控发光成像。这项工作将阐明可见光激发和细胞器靶向镧系元素分子探针的设计，以用于更广泛的时间门控发光应用。