MicroRNAs play a crucial role in sperm formation, but its specific function remains unknown. Here, we found that gga-miR-218 regulates chicken sperm formation through in/ex vivo experiments. We constructed over-expression/interference carrier to overexpress and inhibit gga-miR-218 in chicken spermatogonial stem cells, separately, the detection of haploid and QRT-PCR of meiosis related genes revealed that gga-miR-218 inhibits meiosis. After injection of miR-218 in vivo, semen concentration and HE (Hematoxylin and Eosin staining) revealed that gga-miR-218 inhibits meiosis. Meanwhile, we discovered that gga-miR-218 could target Stra8 by prediction software which can inhibit the wild-type fluorescence activity by co-transfection of gga-miR-218 with the Stra8 3' untranslated regions fluorescent reporter vector (wild-type/mutant), QRT-PCR and Western blot showed that gga-miR-218 inhibits the expression level of Stra8 by targeting its 3' untranslated regions directly. Finally, we suggest that gga-miR-218 could target to srta8 directly and inhibit spermatogenesis.

中文翻译：

---

gga-miR-218通过靶向Stra8调控精原干细胞减数分裂

MicroRNAs 在精子形成过程中起着至关重要的作用，但其具体功能尚不清楚。在这里，我们发现 gga-miR-218 通过体内/体外实验调节鸡精子的形成。我们构建了过表达/干扰载体在鸡精原干细胞中过表达和抑制gga-miR-218，分别通过单倍体检测和减数分裂相关基因的QRT-PCR显示gga-miR-218抑制减数分裂。体内注射 miR-218 后，精液浓度和 HE（苏木精和伊红染色）显示 gga-miR-218 抑制减数分裂。同时，我们发现gga-miR-218可以通过预测软件靶向Stra8，该软件可以通过gga-miR-218与Stra8 3'非翻译区荧光报告载体（野生型/突变体），QRT-PCR 和蛋白质印迹表明 gga-miR-218 通过直接靶向其 3' 非翻译区来抑制 Stra8 的表达水平。最后，我们建议 gga-miR-218 可以直接靶向 srta8 并抑制精子发生。