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Label-free comparative proteomic and physiological analysis provides insight into leaf color variation of the golden-yellow leaf mutant of Lagerstroemia indica.
Journal of Proteomics ( IF 3.3 ) Pub Date : 2020-08-14 , DOI: 10.1016/j.jprot.2020.103942
Sumei Li 1 , Shuan Wang 1 , Peng Wang 1 , Lulu Gao 1 , Rutong Yang 1 , Ya Li 1
Affiliation  

GL1 is a golden-yellow leaf mutant that cultivated from natural bud-mutation of Lagerstroemia indica and has a very low level of photosynthetic pigment under sunlight. GL1 can gradually increase its pigment content and turn into pale-green leaf when shading under sunshade net (referred as Re-GL1). The mechanisms that cause leaf color variation are complicated and are not still unclear. Here, we have used a label-free comparative proteomics to investigate differences in proteins abundance and analyze the specific biological process associated with mechanisms of leaf color variation in GL1. A total of 245 and 160 proteins with different abundance were identified in GL1 vs WT and GL1 vs Re-GL1, respectively. Functional classification analysis revealed that the proteins with different abundance mainly related to photosynthesis, heat shock proteins, ribosome proteins, and oxidation-reduction. The proteins that the most significantly contributed to leaf color variation were photosynthetic proteins of PSII and PSI, which directly related to photooxidation and determined the photosynthetic performance of photosystem. Further analysis demonstrated that low jasmonic acid content was needed to golden-yellow leaf GL1. These findings lay a solid foundation for future studies into the molecular mechanisms that underlie leaf color formation of GL1.

Biological significance

The natural bud mutant GL1 of L. indica is an example through changing leaf color to cope with complex environment. However, the molecular mechanism of leaf color variation are largely elusive. The proteins with different abundance identified from a label-free comparative proteomics revealed a range of biological processes associated with leaf color variation, including photosynthesis, oxidation-reduction and jasmonic acid signaling. The photooxidation and low level of jasmonic acid played a primary role in GL1 adaptation in golden-yellow leaf. These findings provide possible pathway or signal for the molecular mechanism associated with leaf color formation and as a valuable resource for signal transaction of chloroplast.



中文翻译:

无标记的比较蛋白质组学和生理学分析提供了对印度紫薇金黄色叶子突变体的叶子颜色变化的了解。

GL1是金黄色叶子突变体,其是从印度紫薇的自然芽突变培养而来的,在阳光下其光合色素的含量非常低。在遮阳网下遮光时,GL1可以逐渐增加其颜料含量,并变成淡绿色的叶子(称为Re-GL1)。导致叶片颜色变化的机制很复杂,并且还不清楚。在这里,我们使用了无标记的比较蛋白质组学来研究蛋白质丰度的差异,并分析与GL1叶片颜色变化机制相关的特定生物学过程。在GL1 vs WT和GL1中共鉴定了245种和160种不同丰度的蛋白质Re-GL1分别。功能分类分析表明,不同丰度的蛋白质主要与光合作用,热激蛋白,核糖体蛋白和氧化还原有关。引起叶色变化最明显的蛋白是PSII和PSI的光合蛋白,它们直接与光氧化有关,并决定了光系统的光合性能。进一步的分析表明,金黄色叶子GL1需要低茉莉酸含量。这些发现为将来研究GL1叶片颜色形成的分子机制奠定了坚实的基础。

生物学意义

自然芽变GL1紫薇是通过改变叶的颜色,以应付复杂的环境的示例。但是,叶色变化的分子机制在很大程度上难以捉摸。从无标记的比较蛋白质组学中鉴定到的具有不同丰度的蛋白质揭示了一系列与叶片颜色变化相关的生物学过程,包括光合作用,氧化还原和茉莉酸信号传导。茉莉酸的光氧化和低水平在金黄色叶片的GL1适应中起主要作用。这些发现为与叶色形成相关的分子机制提供了可能的途径或信号,并且为叶绿体的信号交换提供了宝贵的资源。

更新日期:2020-08-20
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