Columns of different structures have different potential kinetic performance – the trade-off between separation, time, and pressure. However, the full potential of a structure cannot always be realized in practically existing columns. Each combination of column efficiency, time, and pressure requires certain cross-sectional dimensions of the column flow-through channels. However, there are limits to the narrowest flow-through channels that can be manufactured with current technology. As a result, columns of some structures cannot be optimized for providing the required efficiency in the shortest time. Additionally, the full potential of its structure can be realized only if a column can operate at the highest pressure available from liquid chromatography (LC) equipment, has sufficient loadability, and satisfies other practical requirements.

Equations tailored for a systematic approach to evaluation of factors affecting performance of optimized LC columns (effects of column structure, column dimensions, operational conditions, etc.) were developed. Parameters quantifying the performance of a specific column at and below its largest acceptable pressure were identified. New objective performance parameters of columns and their structures were introduced. Among them are the apparent structural quality factor accounting for the effect of insufficiently high pressure acceptable for the column, the dimensionless plate duration – the parameter of a column structure affecting its performance when the pressure is not limited, – and others.

Applying the theory developed herein to published data, the performance of several differently structured columns is evaluated, and the factors affecting their comparative performance are discussed.

In the final count, not the quality of a column structure, but practical factors such as the narrowest manufacturable flow-through channels can dominate the choice of the kinetically most suitable column for a practical LC analysis.

不同结构的色谱柱具有不同的潜在动力学性能-分离度，时间和压力之间的权衡。但是，在实际存在的色谱柱中无法始终实现结构的全部潜力。色谱柱效率，时间和压力的每种组合都需要色谱柱流通通道的某些横截面尺寸。但是，可以使用当前技术制造的最窄的流通通道受到限制。结果，不能优化某些结构的列以在最短的时间内提供所需的效率。此外，只有当色谱柱可以在液相色谱（LC）设备提供的最高压力下运行，具有足够的负载能力并满足其他实际要求时，才能实现其结构的全部潜能。

开发了针对系统方法而设计的方程式，用于评估影响优化液相色谱柱性能的因素（柱结构，柱尺寸，操作条件等的影响）。确定了量化特定色谱柱在最大可接受压力以下的性能的参数。介绍了新的柱子客观性能参数及其结构。其中包括表观结构品质因数，该因数考虑了色谱柱可接受的压力不足的影响，无因次板的持续时间–不受压力限制时影响其性能的色谱柱结构的参数–以及其他。

将本文开发的理论应用于公开数据，评估了几种结构不同的色谱柱的性能，并讨论了影响其比较性能的因素。

最后，不是最重要的色谱柱结构，而是实际因素（例如最窄的可流通流道）可以决定在动力学上最适合用于实际LC分析的色谱柱的选择。