Praziquantel (PZQ) is a drug commonly used to treat some parasitic infections in animals. This study aimed to apply a reliable and simple method to identify important biological metabolites relevant to PZQ in crucian carp (Carassius auratus) to decipher the metabolic pathways and provide a basis for developing new anti-parasite drugs. The experimental group of crucian carp was administered oral PZQ at a dose of 10 mg kg⁻¹ via a stomach feed tube. All biological blood samples were analysed using liquid chromatography electrospray ionization/quadrupole time-of-flight mass spectrometry (LC ESI/Q-TOF MS). MetPA analysis was used to identify relevant pathways for PZQ in crucian carp. Thirty-five potential metabolic pathways were revealed by MetPA network software. Furthermore, the chemical structures of the related metabolites and pathways were identified by comparison with data obtained from free online databases. Forty-four significantly differentially abundant endogenous metabolites were found in the PZQ-treated crucian carp. The changes in metabolomic profiles and pathways induced by PZQ played a role in inhibiting pathogenesis.

吡喹酮（PZQ）是通常用于治疗动物中某些寄生虫感染的药物。本研究旨在应用可靠和简单的方法来识别与吡喹酮鲫鱼（重要的生物代谢产物鲫鱼）破译的代谢途径，并为开发新的抗寄生虫药物的基础。实验组给carp鱼口服PZQ，剂量为10 mg kg ^-1通过胃饲管。使用液相色谱电喷雾电离/四极杆飞行时间质谱（LC ESI / Q-TOF MS）分析所有生物血样。MetPA分析用于确定cru鱼中PZQ的相关途径。MetPA网络软件揭示了35条潜在的代谢途径。此外，通过与从免费在线数据库获得的数据进行比较，确定了相关代谢产物的化学结构和途径。在PZQ处理的cru鱼中发现了44种差异显着丰富的内源代谢产物。PZQ诱导的代谢组学谱和途径的变化在抑制发病机理中起作用。