Abstract Low temperature is a common environmental stress factor. Polyploidy has often been considered to confer adaptation to cold stress in plants. However, the mechanisms by which abscisic acid (ABA) regulates non-structural carbon (NSC) in polyploids, yielding an advantage in various ecological contexts, are largely unknown. Here, we have compared transcriptomic data from two Fragaria species in response to cold stress to assess ABA-regulated cold tolerance mechanisms. Endogenous ABA and proline content as well as superoxide dismutase (SOD) and peroxidase activities of tetraploid Fragaria moupinensis were higher than those of diploid F.pentaphylla under control conditions. Cold stress significantly increased proline content and SOD activity in both Fragaria species. We found that accumulation of soluble sugar and non-structural carbon under cold stress are key factors underlying cold resistance in F. moupinensis. Under cold stress, compared to F. pentaphylla, ABA-related genes exhibited significant differences in regulation. In contrast, in F. moupinensis, some anti-oxidation genes and transcription factors were up-regulated relative to F. pentaphylla under cold stress. In total, 1274 genes were associated with ABA. Our results revealed up-regulated ABA-related genes, anti-oxidant genes, and transcription factors and the consequent accumulation of NSC to likely underlie cold tolerance in tetraploid F. moupinensis.

中文翻译：

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ABA 调控的倍性相关基因和非结构碳积累可能是四倍体草莓果冻耐寒的基础

摘要 低温是一种常见的环境胁迫因素。多倍体常被认为赋予植物对冷胁迫的适应性。然而，脱落酸 (ABA) 调节多倍体中的非结构碳 (NSC) 的机制，在各种生态环境中产生优势，在很大程度上是未知的。在这里，我们比较了两种草莓属植物响应冷应激的转录组数据，以评估 ABA 调节的耐寒机制。在对照条件下，四倍体草莓果的内源ABA和脯氨酸含量以及超氧化物歧化酶（SOD）和过氧化物酶活性均高于二倍体五叶枫。冷应激显着增加了两种草莓属植物的脯氨酸含量和 SOD 活性。我们发现冷胁迫下可溶性糖和非结构性碳的积累是木平松抗寒的关键因素。在冷胁迫下，与 F. pentaphylla 相比，ABA 相关基因在调控方面表现出显着差异。相比之下，在冷胁迫下，在 F. moupinensis 中，一些抗氧化基因和转录因子相对于 F. pentaphylla 上调。总共有 1274 个基因与 ABA 相关。我们的研究结果揭示了 ABA 相关基因、抗氧化基因和转录因子的上调以及随之而来的 NSC 积累，可能是四倍体 F. moupinensis 耐寒的基础。在冷胁迫下，F. moupinensis 的一些抗氧化基因和转录因子相对于 F. pentaphylla 上调。总共有 1274 个基因与 ABA 相关。我们的研究结果揭示了 ABA 相关基因、抗氧化基因和转录因子的上调以及随之而来的 NSC 积累，可能是四倍体 F. moupinensis 耐寒的基础。在冷胁迫下，F. moupinensis 的一些抗氧化基因和转录因子相对于 F. pentaphylla 上调。总共有 1274 个基因与 ABA 相关。我们的研究结果揭示了 ABA 相关基因、抗氧化基因和转录因子的上调以及随之而来的 NSC 积累，可能是四倍体 F. moupinensis 耐寒的基础。