Abstract A novel disease of mango (Mangifera indica L.) was observed in the main mango growing regions (Minab, Bandar Abbas and Roudan) of Hormozgan province, southern Iran. Disease symptoms included leaf and fruit blights as well as twigs and branches dieback. The disease incidence in the field conditions ranged from 15.3% to 27.3%. A black yeast-like fungus was consistently isolated from symptomatic tissues and identified as Aureobasidium melanogenum using cultural and morphological features as well as partial nucleotide sequence data derived from two gene loci, including internal transcribed spacer region (ITS1–5.8S-ITS2) and D1/D2 domains of the large subunit (26S) (LSU-rRNA) of the ribosomal DNA gene. BLASTn searches of the ITS and LSU sequences in the NCBI GenBank revealed 99–100% nucleotide similarity to several A. melanogenum strains. A phylogeny inferred using sequence data from ITS and LSU sequences placed our isolates together with the other A. melanogenum in the GenBank. Koch's postulates were fulfilled by inoculating stems, leaves and fruits with A. melanogenum isolates. To our knowledge, this is the first report worldwide of A. melanogenum on mango.

中文翻译：

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芒果上新出现的真菌病原体的高分布率：伊朗南部的案例研究

摘要 在伊朗南部霍尔木兹甘省的主要芒果产区（Minab、Bandar Abbas 和 Roudan）发现了一种新的芒果病害（Mangifera indica L.）。病害症状包括叶枯病和果实枯萎病以及树枝和树枝枯死。田间条件下的发病率为15.3%～27.3%。一种黑色酵母样真菌始终从有症状的组织中分离出来，并使用培养和形态特征以及来自两个基因位点的部分核苷酸序列数据，包括内部转录间隔区 (ITS1–5.8S-ITS2) 和 D1，将其鉴定为 Aureobasidium melanogenum核糖体 DNA 基因的大亚基 (26S) (LSU-rRNA) 的 /D2 域。对 NCBI GenBank 中 ITS 和 LSU 序列的 BLASTn 搜索显示，与几个 A. melanogenum 菌株有 99-100% 的核苷酸相似性。使用来自 ITS 和 LSU 序列的序列数据推断的系统发育将我们的分离株与 GenBank 中的其他 A. melanogenum 放在一起。Koch 的假设是通过用 A. melanogenum 分离株接种茎、叶和果实来实现的。据我们所知，这是世界范围内关于芒果的 A. melanogenum 的第一份报告。