To address the availability of carbon sources for denitrification, the accelerated hydrolysis of the most abundant but low-availability fraction of particulate organic matter (POM) was investigated. Mesh sieves with different pore sizes were used as primary pretreatment at the start-up-stage of the biological process to separate some POM from the liquid system. The changes in soluble carbohydrates and proteins were monitored to investigate the hydrolysis performance of the sieved POM, with waste activated sludge (WAS) as the control test. The results showed that an average of 35% POM could be entrapped before filtrate mat development. In addition, benefiting from the high polysaccharides concentration, as well as the high availability due to the relatively loose physical structure, a 23% hydrolysis efficiency of POM was obtained, in contrast to that of WAS (3.4%), with a hydrolysis constant of 0.39 h⁻¹. The prominent performance was also attributed to the unique microbial communities having been domesticated at a lower temperature, especially the cellulose-degrading bacteria Paraclostridium and psychrophile Psychrobacter, making up 6.94% and 2.56%, respectively. Furthermore, the potential benefits and application of improved POM hydrolysis by start-up stage recovery via mesh sieves combined with anaerobic fermentation were evaluated, including selective POM entrapment, alleviation of blockage and wear, and a reduction in aeration energy. By the proposed strategy, carbon availability for biological nutrient removal (BNR) processes is anticipated to be improved more economically than that can be achieved by primary clarifier elimination.

为了解决用于反硝化的碳源的可用性，研究了颗粒有机物（POM）含量最高但利用率低的部分的加速水解。在生物过程的启动阶段，将具有不同孔径的筛孔筛作为主要预处理方法，以从液体系统中分离出一些POM。监测可溶性碳水化合物和蛋白质的变化，以筛分的聚甲醛的水解性能为研究对象，以废活性污泥（WAS）作为对照测试。结果表明，平均35％的POM可以在滤液垫形成之前被捕获。此外，得益于较高的多糖浓度，以及由于相对松散的物理结构而获得的高可用性，POM的水解效率为23％，^-1。杰出的表现还归因于在较低温度下驯化的独特微生物群落，尤其是降解纤维素的细菌副梭菌和嗜冷菌Psychrobacter，分别占6.94％和2.56％。此外，还评估了通过筛网的启动阶段回收与厌氧发酵相结合改善POM水解的潜在益处和应用，包括选择性POM截留，减轻堵塞和磨损以及降低通气能量。通过提出的策略，预计生物去除营养（BNR）过程的碳利用率将比通过消除初级澄清池实现的经济效益更高。