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Bacillus licheniformis BCR 4-3 increases immune response and survival of Litopenaeus vannamei challenged with Vibrio parahaemolyticus IPNGS16
Aquaculture International ( IF 2.9 ) Pub Date : 2020-08-13 , DOI: 10.1007/s10499-020-00585-2
Darío Israel García-Medel , Carlos Angulo , Ruth Escamilla-Montes , Jesús Arturo Fierro-Coronado , Genaro Diarte-Plata , Carina Gámez-Jiménez , Antonio Luna-González

Immunoprophylactic alternatives are needed to fight against acute hepatopancreatic necrosis disease (AHPND). This study addressed the effects of different routes of Bacillus administration (via diet or culture water) on growth performance, survival, and immune parameters of Litopenaeus vannamei challenged with Vibrio parahaemolyticus-AHPND. Bacillus mixture and Bacillus licheniformis BCR 4-3 were added to feed (1 × 106 CFU g−1) or rearing water (1 × 106, 2 × 106, 3 × 106 CFU L−1). Animals were challenged with a determined median lethal V. parahaemolyticus concentration. Survival, growth performance, and immune response were determined. The results showed that B. licheniformis BCR 4-3 (3 × 106 CFU L−1) administration via culture water significantly (P < 0.05) increased survival (53.4%) in shrimp challenged with V. parahaemolyticus; growth performance was also significantly (P < 0.05) higher compared to the control group. Moreover, the relative expression of innate immune-related genes, such as lysozyme, penaeidin4, crustin, and superoxide dismutase, significantly (P < 0.05) upregulated in shrimp fed B. licheniformis BCR4-3 compared with the control group. In conclusion, the administration of probiotic B. licheniformis BCR4-3 in feed and culture water increased resistance in L. vannamei against V. parahaemolyticus-AHPND and was found associated with an upregulation of key antimicrobial innate immune-related genes.



中文翻译:

地衣芽孢杆菌BCR 4-3可提高副溶血性弧菌IPNGS16攻击的南美白对虾的免疫应答和存活率

需要使用免疫预防替代品来对抗急性肝胰腺坏死病(AHPND)。这项研究探讨了不同途径的芽孢杆菌施用途径(通过饮食或培养水)对用副溶血性弧菌-AHPND攻击的南美白对虾的生长性能,存活率和免疫参数的影响。混合物和芽孢杆菌地衣芽孢杆菌BCR 4-3加入到进料(1×10 6  CFU克-1)或饲养用水(1×10 6,2×10 6,3×10 6  CFU大号-1)。用确定的中等致死性副溶血弧菌攻击动物浓度。确定存活率,生长性能和免疫反应。结果表明,通过培养水施用地衣芽孢杆菌BCR 4-3(3×10 6  CFU L -1)可显着(P  <0.05)增加副溶血弧菌对虾的存活率(53.4%); 与对照组相比,生长性能也显着提高(P <0.05)。此外,在地衣芽孢杆菌 对虾饲喂的虾中,固有的免疫相关基因(如溶菌酶,Penaeidin4,Crustin和超氧化物歧化酶)的相对表达显着上调(P <0.05)BCR4-3与对照组比较。总之,益生菌的管理地衣芽孢杆菌的饲料BCR4-3和养殖水阻力增大南美白对虾副溶血性弧菌-AHPND,并发现与关键抗菌先天免疫相关基因的上调有关。

更新日期:2020-08-14
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