Significance: Diffuse optical spectroscopic imaging (DOSI) measures quantitative functional and molecular information in thick tissue in a noninvasive manner using near-infrared light. DOSI may be useful for diagnosis and prognosis of bone pathologies including osteosarcoma and Ewing’s sarcoma, but little is currently known about DOSI-derived parameters in bony anatomic locations where this disease occurs. Aim: Our goal is to quantify the optical characteristics and chromophore content of bony anatomic locations of healthy volunteers and assess differences due to anatomic region, age, sex, ethnicity, race, and body fat. Approach: Fifty-five healthy volunteers aged 4 to 72 were enrolled in the study. The optical properties and quantitative tissue concentrations of oxyhemoglobin, deoxyhemoglobin, water, and lipids were assessed at the distal humerus, distal femur, and proximal tibia. Body fat was assessed using skinfold calipers. One volunteer underwent a more comprehensive body scan from neck to foot to explore chromophore distributions within an individual. Regression analysis was used to identify the most important sources of variation in the measured data set. Results: Statistical differences between bony locations were found for scattering, water, and lipids, but not for hemoglobin. All chromophores had statistical differences with sex, but there were no significant age-related correlations. Regression analysis revealed that body fat measured with skinfold calipers was the most important predictor of oxy-, deoxy-, total hemoglobin, and lipids. Hemoglobin and lipid levels were highly correlated (ρ ≥ 0.7) over the subject population and within the single-subject body scan. Conclusions: DOSI can successfully measure bony anatomic sites where osteosarcomas and Ewing’s sarcomas commonly occur. Future studies of bone pathology using DOSI should account for the variation caused by anatomic region, sex, race and ethnicity, and body fat as these cause substantial variations in DOSI-derived metrics.

中文翻译：

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使用漫反射光谱成像表征儿童和成人健康志愿者的骨解剖区域。

意义：漫射光谱成像 (DOSI) 使用近红外光以非侵入性方式测量厚组织中的定量功能和分子信息。DOSI 可用于骨病变的诊断和预后，包括骨肉瘤和尤文氏肉瘤，但目前对发生这种疾病的骨解剖位置的 DOSI 衍生参数知之甚少。目的：我们的目标是量化健康志愿者骨骼解剖位置的光学特征和发色团含量，并评估由于解剖区域、年龄、性别、种族、种族和体脂造成的差异。方法：55 名 4 至 72 岁的健康志愿者参加了这项研究。在肱骨远端评估氧合血红蛋白、脱氧血红蛋白、水和脂质的光学特性和定量组织浓度，股骨远端和胫骨近端。使用皮褶卡尺评估体脂。一名志愿者接受了从颈部到足部的更全面的身体扫描，以探索个体内的发色团分布。回归分析用于确定测量数据集中最重要的变异来源。结果：在散射、水和脂质方面发现了骨位置之间的统计差异，但在血红蛋白方面没有发现。所有发色团都与性别有统计学差异，但没有显着的年龄相关性。回归分析显示，用皮褶卡尺测量的体脂是氧、脱氧、总血红蛋白和脂质的最重要预测因子。血红蛋白和脂质水平在受试者群体和单受试者身体扫描中高度相关 (ρ ≥ 0.7)。结论：DOSI 可以成功测量骨肉瘤和尤文肉瘤常见的骨解剖部位。未来使用 DOSI 进行的骨病理学研究应该考虑由解剖区域、性别、种族和民族以及体脂引起的变化，因为这些会导致 DOSI 衍生指标的显着变化。