Abstract

Leptin is a hormone synthesized and secreted primarily in adipocyte which can help to regulate energy balance. In this experiment, three tissue samples of Tianfu black rabbits at four growing periods were selected. The expression levels of leptin gene in different tissues were detected by real-time fluorescence quantitative PCR. The correlation analysis showed that the correlation coefficient between the expression levels of leptin gene in perirenal fat and intramuscular fat content in 84-day-old male rabbits was 0.73 (p < 0.05); the correlation coefficients between the expression levels of leptin gene in left biceps femoris and intramuscular fat and 24-hour pH in 84-day-old male rabbits were 0.95 (p < 0.01) and 0.85 (p < 0.05), respectively. Besides, the correlation coefficient between the expression levels of leptin gene in male left biceps femoris and cooked meat rate was 0.83 (p < 0.05). According to the analysis results, we inferred the expression levels of leptin gene in Tianfu black rabbits can influence meat quality and the meat quality of high expression levels of leptin gene in Tianfu black rabbits is better. These results revealed the leptin gene may be one of the important candidate genes for meat quality traits of molecular markers.

摘要

瘦素是一种主要在脂肪细胞中合成和分泌的激素，有助于调节能量平衡。本实验选取天府黑兔4个生长期的3个组织样本。实时荧光定量PCR检测瘦素基因在不同组织中的表达水平。相关性分析显示，84日龄雄性家兔肾周脂肪瘦素基因表达水平与肌内脂肪含量的相关系数为0.73（ p  < 0.05）；84日龄雄性家兔左侧股二头肌瘦素基因表达水平与肌内脂肪与24小时pH的相关系数为0.95（ p < 0.01) 和 0.85 ( p  < 0.05)，分别。此外，男性左股二头肌瘦素基因表达水平与熟肉率的相关系数为0.83（p  < 0.05）。根据分析结果推断，瘦素基因在天府黑兔中的表达水平会影响肉质，瘦素基因在天府黑兔中高表达的肉质较好。这些结果表明瘦素基因可能是肉质性状分子标记的重要候选基因之一。