Cisplatin is an effective anticancer drug used against a variety of cancers. The full therapeutic potential of cisplatin is often hampered due to concurrent development of various side effects in the hosts. Rutin, a naturally occurring bioflavonoid shows several pharmacological activities. It has been earlier reported by us that rutin and cisplatin in combination show better antitumor activity against murine ascites Dalton’s lymphoma. As cisplatin is given to cancer-bearing hosts only, the present study was undertaken to explore the histoprotective effect of rutin against some toxicities induced by cisplatin in tumor-bearing mice. Cisplatin treatment caused severe damages in tissue architecture such as degenerated hepatocytes with nuclear condensation and sinusoidal dilatation in the liver, glomerular deterioration, infiltration of cells, and tubular congestion in the kidney, and vacuolization of Sertoli cells or dense granules in the cytoplasm and damaged seminiferous tubules in the testes. In the rutin plus cisplatin combination-treated mice, all the abnormal tissue architectural features were decreased. Further, as compared to cisplatin treatment, combination treatment did not show any significant elevation in the liver functional biomarkers (serum aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase) and renal functional biomarkers (serum urea and creatinine levels). The combination treatment reduced the sperm abnormalities also as compared to the cisplatin alone treatment. The in vitro hemolysis assay of red blood cells and scanning electron microscopy revealed that combination treatment lessened the cisplatin-induced hemolysis and abnormalities in RBCs. Thus, the present findings demonstrate that rutin has histoprotective ability against cisplatin-induced toxicities in tumor-bearing mice.

顺铂是一种有效的抗癌药物，可用于对抗多种癌症。由于在宿主中同时发生各种副作用，顺铂的全部治疗潜力常常受到阻碍。芦丁是一种天然存在的生物类黄酮，具有多种药理活性。我们早先报道过芦丁和顺铂联合应用对鼠腹水道尔顿淋巴瘤显示出更好的抗肿瘤活性。由于顺铂仅用于荷癌宿主，本研究旨在探讨芦丁对顺铂在荷瘤小鼠中诱导的某些毒性的组织保护作用。顺铂治疗对组织结构造成严重损害，例如退化的肝细胞，伴有核浓缩和肝窦扩张、肾小球退化、细胞浸润、肾小管充血，细胞质内支持细胞空泡化或致密颗粒，睾丸生精小管受损。在芦丁加顺铂联合治疗的小鼠中，所有异常的组织结构特征都减少了。此外，与顺铂治疗相比，联合治疗未显示肝功能生物标志物（血清天冬氨酸氨基转移酶、丙氨酸氨基转移酶和碱性磷酸酶）和肾功能生物标志物（血清尿素和肌酐水平）的任何显着升高。与单独的顺铂治疗相比，联合治疗也减少了精子异常。这 在芦丁加顺铂联合治疗的小鼠中，所有异常的组织结构特征都减少了。此外，与顺铂治疗相比，联合治疗未显示肝功能生物标志物（血清天冬氨酸氨基转移酶、丙氨酸氨基转移酶和碱性磷酸酶）和肾功能生物标志物（血清尿素和肌酐水平）的任何显着升高。与单独的顺铂治疗相比，联合治疗也减少了精子异常。这 在芦丁加顺铂联合治疗的小鼠中，所有异常的组织结构特征都减少了。此外，与顺铂治疗相比，联合治疗未显示肝功能生物标志物（血清天冬氨酸氨基转移酶、丙氨酸氨基转移酶和碱性磷酸酶）和肾功能生物标志物（血清尿素和肌酐水平）的任何显着升高。与单独的顺铂治疗相比，联合治疗也减少了精子异常。这 丙氨酸氨基转移酶和碱性磷酸酶）和肾功能生物标志物（血清尿素和肌酐水平）。与单独的顺铂治疗相比，联合治疗也减少了精子异常。这 丙氨酸氨基转移酶和碱性磷酸酶）和肾功能生物标志物（血清尿素和肌酐水平）。与单独的顺铂治疗相比，联合治疗也减少了精子异常。这红细胞的体外溶血测定和扫描电子显微镜显示，联合治疗减轻了顺铂诱导的溶血和红细胞异常。因此，本研究结果表明芦丁对荷瘤小鼠中顺铂诱导的毒性具有组织保护能力。