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Insights into Protein Stability in Cell Lysate by 19 F NMR Spectroscopy.
ChemBioChem ( IF 3.2 ) Pub Date : 2020-08-12 , DOI: 10.1002/cbic.202000413
Hannah Welte 1 , Michael Kovermann 1
Affiliation  

The importance of biological environment: Fluorine NMR spectroscopy was applied to determine the thermodynamic stability, ΔG0, of 19F‐labeled cold shock protein B, BsCspB, during fully reversible folding‐to‐unfolding transitions in cell lysate at different cell pellet concentrations.
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中文翻译:

通过 19 F NMR 光谱深入了解细胞裂解物中的蛋白质稳定性。

生物环境的重要性:应用氟核磁共振波谱法测定19 F 标记的冷休克蛋白 B ( Bs CspB ) 在不同细胞沉淀的细胞裂解液完全可逆折叠到解折叠转变期间的热力学稳定性 Δ G 0浓度。
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更新日期:2020-08-12
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