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Correlation between intermediary metabolism, Hsp gene expression, and oxidative stress-related proteins in long-term thermal-stressed Mytilus galloprovincialis.
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology ( IF 2.8 ) Pub Date : 2020-08-13 , DOI: 10.1152/ajpregu.00066.2020
Konstantinos Feidantsis 1 , Ioannis A Giantsis 2 , Andreas Vratsistas 1 , Stavroula Makri 1 , Athanasia-Zoi Pappa 1 , Elena Drosopoulou 3 , Andreas Anestis 4 , Evangelia Mavridou 1 , Athanasios Exadactylos 5 , Dimitrios Vafidis 5 , Basile Michaelidis 1
Affiliation  

Long-term exposure of Mytilus galloprovincialis to temperatures beyond 26°C triggers mussel mortality. The present study aimed to integratively illustrate the correlation between intermediary metabolism, hsp gene expression, and oxidative stress-related proteins in long-term thermally stressed Mytilus galloprovincialis and whether they are affected by thermal stress magnitude and duration. We accordingly evaluated the gene expression profiles, in the posterior adductor muscle (PAM) and the mantle, concerning heat shock protein 70 and 90 (hsp70 and hsp90), and the antioxidant defense indicators Mn-SOD, Cu/Zn-SOD, catalase, glutathione S-transferase, and the metallothioneins mt-10 and mt-20. Moreover, we determined antioxidant enzyme activities, oxidative stress through lipid peroxidation, and activities of intermediary metabolism enzymes. The pattern of changes in relative mRNA expression levels indicate that mussels are able to sense thermal stress even when exposed to 22°C and before mussel mortality is initiated. Data indicate a close correlation between the magnitude and duration of thermal stress with lipid peroxidation levels and changes in the activity of antioxidant enzymes and the enzymes of intermediary metabolism. The gene expression and increase in the activities of antioxidant enzymes support a scenario, according to which exposure to 24°C might trigger reactive oxygen species (ROS) production, which is closely correlated with anaerobic metabolism under hypometabolic conditions. Increase and maintenance of oxidative stress in conjunction with energy balance disturbance seem to trigger mussel mortality after long-term exposure at temperatures beyond 26°C. Eventually, in the context of preparation for oxidative stress, certain hypotheses and models are suggested, integrating the several steps of cellular stress response.

中文翻译:

长期热应激的Mytilus galloprovincialis中间代谢,Hsp基因表达和氧化应激相关蛋白之间的相关性。

的长期暴露紫贻贝到超过26℃的触发器贻贝死亡率温度。本研究旨在综合说明长期受热应激的Mytilus galloprovincialis中间代谢,hsp基因表达和氧化应激相关蛋白之间的相关性,以及它们是否受热应激强度和持续时间的影响。因此,我们评估了后内收肌(PAM)和地幔中与热休克蛋白70和90(hsp70hsp90)有关的基因表达谱,以及抗氧化剂防御指标Mn-SOD,Cu / Zn-SOD过氧化氢酶谷胱甘肽S-转移酶和金属硫蛋白mt-10mt-20。此外,我们确定了抗氧化酶的活性,脂质过氧化引起的氧化应激以及中间代谢酶的活性。相对mRNA表达水平的变化模式表明,贻贝即使在暴露于22°C时和贻贝死亡开始之前也能够感知热应激。数据表明,热应激的大小和持续时间与脂质过氧化水平以及抗氧化酶和中间代谢酶的活性变化密切相关。基因表达和抗氧化酶活性的增加支持了一种情况,根据这种情况,暴露于24°C可能触发活性氧(ROS)的产生,这与低代谢条件下的厌氧代谢密切相关。在超过26°C的温度下长期暴露后,氧化应激的增加和维持以及能量平衡紊乱似乎会触发贻贝死亡。最终,在准备氧化应激的背景下,提出了某些假设和模型,整合了细胞应激反应的几个步骤。
更新日期:2020-08-20
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