Elucidation of the underlying mechanisms of plant growth promotion of rhizobacteria is very important. This study explored the mechanism by which Bacillus pumilus LZP02 promotes growth in rice roots through proteomic, transcriptomic, and metabolomic techniques. The results showed that B. pumilus LZP02 promoted the absorption of phosphorous, calcium, and magnesium ions by colonization of rice roots and enhanced peroxidase, catalase, superoxide dismutase, and Ca²⁺Mg²⁺ adenosine triphosphatase activities and chlorophyll contents in rice. The proteomic results showed that most of the differentially expressed proteins were involved in carbohydrate metabolism and that the biosynthesis of other secondary metabolites was also increased. According to RNA-seq and reverse transcription-quantitative PCR analyses, expression of some genes involved in carbohydrate metabolism and phenylpropanoid biosynthesis was upregulated in rice roots. Regarding metabolomics, phenylpropanoid biosynthesis, starch and sucrose metabolism, the pentose phosphate pathway, and glyoxylate and dicarboxylate metabolism were increased. The results indicated that B. pumilus LZP02 promoted the growth of rice roots by enhancing carbohydrate metabolism and phenylpropanoid biosynthesis.

阐明根际细菌促进植物生长的潜在机制非常重要。这项研究探讨了短小芽孢杆菌LZP02通过蛋白质组学，转录组学和代谢组学技术促进水稻根系生长的机制。结果表明，短小芽孢杆菌LZP02通过在水稻根部定植而促进了磷，钙和镁离子的吸收，并增强了过氧化物酶，过氧化氢酶，超氧化物歧化酶和Ca ²⁺ Mg ²⁺水稻中的腺苷三磷酸酶活性和叶绿素含量。蛋白质组学结果表明，大多数差异表达的蛋白质都参与了碳水化合物的代谢，其他次生代谢产物的生物合成也有所增加。根据RNA-seq和逆转录定量PCR分析，水稻根系中参与碳水化合物代谢和苯丙烷生物合成的某些基因的表达上调。关于代谢组学，苯丙氨酸类生物合成，淀粉和蔗糖代谢，磷酸戊糖途径以及乙醛酸和二羧酸酯代谢增加。结果表明，短小芽孢杆菌LZP02通过增强碳水化合物的代谢和苯丙烷的生物合成，促进了水稻根的生长。