All of the known porcine sapeloviruses (PSVs) currently belong to a single genotype in the genus Sapelovirus (family Picornaviridae). Here, the complete genome of a second, possibly recombinant, genotype of PSV strain SZ1M-F/PSV/HUN2013 (MN807752) from a faecal sample of a paraplegic pig in Hungary was characterized using viral metagenomics and RT-PCR. This sapelovirus strain showed only 64 % nucleotide identity in the VP1 region to its closest PSV-1 relative. Complete VP1 sequence-based epidemiological investigations of PSVs circulating in Hungary showed the presence of diverse strains found in high prevalence in enteric and respiratory samples collected from both asymptomatic and paraplegic pigs from 12 swine farms. Virus isolation attempts using PK-15 cell cultures were successful in 3/8 cases for the classic but not the novel PSV genotype. Sequence comparisons of faeces and isolate strains derived VP1 showed that cultured PSV strains not always represent the dominant PSVs found in vivo.

中文翻译：

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匈牙利养猪场的肠道和呼吸道样本中沙门氏菌甲病毒（Picornaviridae）的高流行，遗传多样性和潜在的新基因型。

目前，所有已知的猪细小病毒（PSV）都属于Sapelovirus属（Picornaviridae家族）中的单个基因型。）。在这里，使用病毒宏基因组学和RT-PCR表征了来自匈牙利截瘫猪粪便样本的PSV毒株SZ1M-F / PSV / HUN2013（MN807752）的第二种可能是重组基因型的完整基因组。该细小病毒株在VP1区域中与其最接近的PSV-1亲缘关系仅显示64％的核苷酸同一性。在匈牙利进行的基于VP1序列的完整流行病学调查显示，从12个养猪场的无症状和截瘫猪身上收集的肠道和呼吸道样品中，普遍存在多种菌株。对于经典而非新颖的PSV基因型，使用PK-15细胞培养进行病毒分离的尝试在3/8例中获得了成功。体内。