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Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques.
Archives of Virology ( IF 2.7 ) Pub Date : 2020-08-05 , DOI: 10.1007/s00705-020-04737-w
Mariano Carossino 1 , Hon S Ip 2 , Juergen A Richt 3 , Kendra Shultz 1 , Kimberly Harper 1 , Alan T Loynachan 4 , Fabio Del Piero 1 , Udeni B R Balasuriya 1
Affiliation  

In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.



中文翻译:

通过 RNAscope® 原位杂交和免疫组织化学技术检测 SARS-CoV-2。

原位杂交 (ISH) 和免疫组织化学 (IHC) 是在自然和实验感染动物中表征 SARS-CoV-2 感染和趋向性以及用于诊断目的的重要工具。在这里,我们描述了三种基于 RNAscope ®的针对 ORF1ab、spike 和核衣壳基因的 ISH 检测,以及针对 SARS-CoV-2 的穗蛋白和核衣壳蛋白的 IHC 检测。

更新日期:2020-09-12
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