The full potential of polyketide discovery has yet to be reached owing to a lack of suitable technologies and knowledge required to advance engineering of polyketide biosynthesis. Recent investigations on the discovery, enhancement, and non-natural use of these biosynthetic gene clusters via computational biology, metabolic engineering, structural biology, and enzymology-guided approaches have facilitated improved access to designer polyketides. Here, we discuss recent successes in gene cluster discovery, host strain engineering, precursor-directed biosynthesis, combinatorial biosynthesis, polyketide tailoring, and high-throughput synthetic biology, as well as challenges and outlooks for rapidly generating useful target polyketides.

中文翻译：

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合成生物学能够获得设计的聚酮化合物。

由于缺乏推进聚酮化合物生物合成工程所需的合适技术和知识，聚酮化合物发现的全部潜力尚未发挥。最近通过计算生物学、代谢工程、结构生物学和酶学引导方法对这些生物合成基因簇的发现、增强和非自然使用的研究促进了对设计聚酮化合物的获取。在这里，我们讨论了最近在基因簇发现、宿主菌株工程、前体导向生物合成、组合生物合成、聚酮化合物定制和高通量合成生物学方面的成功，以及快速生成有用的目标聚酮化合物的挑战和前景。