Aminoglycosides are a relevant class of antibiotics widely used by medics and veterinaries. There are a variety of reasons that make their determination relevant, such as quality control, environment and food contamination assessment, drug‐release studies, among others. The lack of a chromophore makes aminoglycoside spectrophotometric detection particularly challenging, often requiring derivatization. In this work, an indirect detection method, making use of imidazole as a probe, applying CZE was successfully tested. It did not require derivatization, which simplified the sample preparation. Suitable figures of merit were obtained; recoveries between 95 and 105%, adequate repeatability and precision, correlation coefficients (r) above 0.998, and limits of detection (LODs) of 3.2 and 11 mg/L for gentamicin and paromomycin, respectively. As a proof‐of‐concept, it was also applied in a simple controlled release experiment that was well fitted using the Hill equation.

中文翻译：

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通过CZE-UV对药物制剂中的氨基糖苷进行无衍生化测定。

氨基糖苷类是医务人员和兽医广泛使用的一类相关抗生素。有多种原因使它们的确定有意义，例如质量控制，环境和食品污染评估，药物释放研究等。发色团的缺乏使得氨基糖苷分光光度法的检测特别具有挑战性，通常需要进行衍生化。在这项工作中，成功地测试了使用咪唑作为探针，采用CZE的间接检测方法。它不需要衍生化，从而简化了样品制备。获得了合适的功绩。回收率在95％至105％之间，具有足够的重复性和精度，相关系数（r）高于0.998，庆大霉素和巴龙霉素的检出限（LOD）分别为3.2和11 mg / L。作为概念验证，它也被用于简单的控释实验中，该实验使用Hill方程很好地拟合了。