Microcrystal electron diffraction (MicroED) was first coined and developed in 2013 at the Janelia Research Campus as a new modality in electron cryomicroscopy (cryoEM). Since then, MicroED has not only made important contributions in pushing the resolution limits of cryoEM protein structure characterization but also of peptides, small-organic and inorganic molecules, and natural-products that have resisted structure determination by other methods. This review showcases important recent developments in MicroED, highlighting the importance of the technique in fields of studies beyond protein structure determination where MicroED is beginning to have paradigm shifting roles.

中文翻译：

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超越使用 MicroED 测定蛋白质结构。

微晶电子衍射 (MicroED) 于 2013 年在 Janelia 研究园区首次创造和开发，作为电子冷冻显微镜 (cryoEM) 的一种新模式。从那时起，MicroED 不仅在突破冷冻电镜蛋白质结构表征的分辨率极限方面做出了重要贡献，而且在其他方​​法无法确定结构的肽、小有机和无机分子以及天然产物方面也做出了重要贡献。这篇综述展示了 MicroED 的最新重要进展，强调了该技术在蛋白质结构测定以外的研究领域的重要性，其中 MicroED 开始发挥范式转变的作用。