Multicellular organisms develop from a single cell, the zygote. This feature is referred to as totipotency. In the mouse, only the zygote and the 2-cell stage embryo display this attribute. Cells resembling the embryonic 2-cell stage blastomeres were identified in embryonic stem (ES) cell cultures as '2-cell-like cells' (2CLCs). This discovery brought the first cellular model with the possibility to investigate some features of the totipotent embryo and the molecular mechanisms regulating totipotency in vitro. In this article, we discuss the latest advancements on the research on 2CLCs, which have uncovered an intricate reprogramming process regulated by proteins as well as metabolites and ncRNAs. These recent findings have shed light on the combinatorial regulation of 2-cell-like cell emergence and the nature of their unique attributes.

中文翻译：

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手中的一个细胞在胚胎中相当于两个：2-细胞样细胞重编程的最新进展。

多细胞生物从单个细胞，即受精卵发育而来。该特征被称为全能性。在小鼠中，只有受精卵和 2 细胞期胚胎显示此属性。类似于胚胎 2 细胞阶段卵裂球的细胞在胚胎干 (ES) 细胞培养物中被鉴定为“2 细胞样细胞”(2CLC)。这一发现带来了第一个细胞模型，有可能研究全能胚胎的一些特征和体外调节全能性的分子机制。在本文中，我们讨论了 2CLC 研究的最新进展，这些进展揭示了由蛋白质、代谢物和 ncRNA 调控的复杂重编程过程。这些最近的发现阐明了 2 细胞样细胞出现的组合调节及其独特属性的性质。