Expression profile analysis and stability evaluation of 18 small RNAs in the Chinese Han population.,Electrophoresis

当前位置： X-MOL 学术 › Electrophoresis › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Expression profile analysis and stability evaluation of 18 small RNAs in the Chinese Han population.
Electrophoresis ( IF 2.9 ) Pub Date : 2020-08-08 , DOI: 10.1002/elps.202000058
Shouyu Wang ₁ , Ruiyang Tao _{1,

2} , Tianyue Ming ₁ , Mengge Wang ₁ , Jing Liu ₁ , Guanglin He ₁ , Xing Zou ₁ , Zheng Wang ₁ , Yiping Hou ₁

Affiliation

In recent years, differentially expressed small RNAs have been widely used to identify the compositions of forensically relevant biological samples, and a vast number of such RNA candidates have been proposed. Nevertheless, when assessing the expression levels of target small RNAs using relative quantitative analysis methods, credible internal controls are usually required for reliable data normalization. Therefore, the identification of optimal reference genes is an important task. In this study, the expression profile of 18 small RNA reference genes was characterized in the Chinese Han population using TaqMan real‐time quantitative PCR. Systematic evaluations of these candidate genes were performed based on their expression levels and stability in several common types of body fluids (i.e., venous blood, menstrual blood, saliva, semen, and vaginal secretions). Analysis results from the ΔCq method, BestKeeper, NormFinder, and geNorm were integrated by RefFinder for ranking and comparing the candidates in each type of body fluid. Among all the candidates, miR‐191 was identified as the most suitable reference gene because it had a favorable ranking value in all tested samples. In addition, miR‐423, miR‐93, miR‐484, and let‐7i were also shown to be applicable reference genes. Overall, this study provides detailed assessment results of these candidate genes in different body fluids; thus, it could be used as a guide for the selection of reference genes according to their performance in the sample of choice.

更新日期：2020-08-08

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南

全部期刊列表>>