Background: Apigenin, a natural plant flavone, has been shown to possess a variety of biological properties.

Objective: In this report, a highly selective and sensitive LC-MS/MS method was developed and validated for the determination of apigenin in rat plasma.

Methods: Analysts were separated on the HSS T3 column (1.8 μm 2.1×100 mm) using acetonitrile and 0.1% formic acid in 2mM ammonium acetate buffer at a supply rate of 0.200 mL/min as eluent in gradient model.

Results: Plasma samples were treated by protein precipitation using acetonitrile for the recovery ranging from 86.5% to 90.1% for apigenin. The calibration curves followed linearity in the concentration range of 0.50-500ng/mL. The inter-day and intra-day precisions at different QC levels within 13.1% and the accuracies ranged from -10.6% to 8.6%.

Conclusion: The assay has been successfully applied to the pharmacokinetic study of apigenin in rats.

背景：芹菜素是一种天然植物黄酮，已被证明具有多种生物学特性。

目的：在本报告中，开发了一种高度选择性和灵敏的LC-MS / MS方法，并已用于测定大鼠血浆中芹菜素的有效性。

方法：在HSS T3色谱柱（1.8μm，2.1×100 mm）上，使用乙腈和0.1％甲酸的2mM醋酸铵缓冲液，以0.200 mL / min的流速作为梯度模型中的洗脱液分离分析物。

结果：血浆样品使用乙腈进行蛋白沉淀处理，芹菜素的回收率在86.5％至90.1％之间。校准曲线在0.50-500ng / mL的浓度范围内呈线性关系。不同质控水平下的日间和日内精度在13.1％以内，准确度范围从-10.6％到8.6％。

结论：该方法已成功应用于芹菜素在大鼠体内的药代动力学研究。