AIM To evaluate the biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on two glass and glass-ceramic materials. Calcium hydroxide (Ca(OH)2 ) paste was used as the positive control. METHODOLOGY The glass-ceramic powder [two-phased Biosilicate (BS-2P)] and F18 bioactive glass were mixed with distilled water (ratio 2 : 1), inserted in polyethylene tubes and implanted in the subcutaneous tissues of 16 rats. Empty tubes were used as negative control. After 7 and 30 days (n = 8), the rats were euthanized for haematoxylin-eosin, von Kossa, polarized light and osteopontin (OPN) immunolabeling analysis. Direct contact tests using a suspension of each paste were performed with Enterococcus faecalis planktonic cells to evaluate antimicrobial activity (24 h of contact), in a pilot study. The number of CFU mL-1 was calculated for each group. The antimicrobial analysis data were submitted to one-way anova and Tukey tests, whilst biocompatibility and immunohistochemical data were submitted to the Kruskal-Wallis and Dunn tests (P < 0.05). RESULTS Most specimens of the control, BS-2P and Ca(OH)2 groups were associated with moderate inflammation seven days following implantation, whilst F18 was associated with moderate to severe inflammation, without differences amongst the groups (P > 0.05). At 30 days, most specimens of control, F18 and BS-2P groups had mild inflammation, whilst Ca(OH)2 had mild to moderate inflammation; however, no differences were determined amongst the groups (P > 0.05). The fibrous capsule was thick at 7 days, becoming thin at 30 days. All pastes induced von Kossa-positive structures and were birefringent to polarized light. At seven days, the BS-2P group had significantly more OPN immunolabeling compared to the control and Ca(OH)2 groups (P < 0.05). At 30 days, the F18 group had significantly more OPN immunolabeling compared to the control and Ca(OH)2 groups (P < 0.05). All pastes reduced the total number of E. faecalis; however, the reduction was only significant when comparing BS-2P and Ca(OH)2 groups to the control (P < 0.05). Only calcium hydroxide eliminated E. faecalis. CONCLUSIONS Experimental BS-2P and F18 pastes were biocompatible, stimulated biomineralization and induced significant OPN immunolabeling compared to Ca(OH)2 . Only the BS-2P paste demonstrated antimicrobial activity comparable to Ca(OH)2 .

中文翻译：

---

基于玻璃和玻璃陶瓷材料的实验管内糊剂的生物相容性，矿化诱导和抗菌活性。

目的评估基于两种玻璃和玻璃陶瓷材料的实验管内糊剂的生物相容性，诱导矿化作用和抗菌活性。氢氧化钙（Ca（OH）2）糊剂用作阳性对照。方法将玻璃陶瓷粉末[两相生物硅酸盐（BS-2P）]和F18生物活性玻璃与蒸馏水混合（比例为2：1），插入聚乙烯管中，并植入16只大鼠的皮下组织中。空管用作阴性对照。7天和30天（n = 8）后，对大鼠进行苏木精-伊红，von Kossa，偏振光和骨桥蛋白（OPN）免疫标记分析。在一项初步研究中，使用粪便肠球菌的浮游细胞进行了每种糊剂悬浮液的直接接触试验，以评估其抗菌活性（接触24小时）。计算每组的CFU mL-1数。抗菌分析数据已提交至单向方差分析和Tukey测试，而生物相容性和免疫组化数据已提交至Kruskal-Wallis和Dunn测试（P <0.05）。结果对照组，BS-2P和Ca（OH）2组的大多数标本在植入后7天与中度炎症有关，而F18与中度至严重炎症相关，各组之间无差异（P> 0.05）。在第30天，对照组，F18和BS-2P组的大多数标本都有轻度炎症，而Ca（OH）2有轻度到中度炎症。但是，各组之间没有差异（P> 0.05）。纤维囊在第7天变稠，在第30天变薄。所有糊剂均诱导了von Kossa阳性结构，并且对偏振光呈双折射。在第7天，与对照组和Ca（OH）2组相比，BS-2P组具有显着更多的OPN免疫标记（P <0.05）。在第30天，与对照组和Ca（OH）2组相比，F18组具有显着更多的OPN免疫标记（P <0.05）。所有糊剂均减少粪肠球菌的总数；然而，只有将BS-2P组和Ca（OH）2组与对照组进行比较时，这种减少才有意义（P <0.05）。仅氢氧化钙消除了粪肠球菌。结论实验性BS-2P和F18糊剂具有生物相容性，可刺激生物矿化并与Ca（OH）2相比具有显着的OPN免疫标记作用。仅BS-2P糊剂显示出与Ca（OH）2相当的抗菌活性。与对照组和Ca（OH）2组相比，BS-2P组的OPN免疫标记明显更多（P <0.05）。在第30天，与对照组和Ca（OH）2组相比，F18组具有显着更多的OPN免疫标记（P <0.05）。所有糊剂均减少粪肠球菌的总数；但是，只有将BS-2P组和Ca（OH）2组与对照组进行比较时，这种减少才有意义（P <0.05）。仅氢氧化钙消除了粪肠球菌。结论实验性BS-2P和F18糊剂具有生物相容性，可刺激生物矿化并与Ca（OH）2相比具有显着的OPN免疫标记作用。仅BS-2P糊剂显示出与Ca（OH）2相当的抗菌活性。与对照组和Ca（OH）2组相比，BS-2P组的OPN免疫标记明显更多（P <0.05）。在第30天，与对照组和Ca（OH）2组相比，F18组具有显着更多的OPN免疫标记（P <0.05）。所有糊剂均减少粪肠球菌的总数；然而，只有将BS-2P组和Ca（OH）2组与对照组进行比较时，这种减少才有意义（P <0.05）。仅氢氧化钙消除了粪肠球菌。结论实验性BS-2P和F18糊剂具有生物相容性，可刺激生物矿化并与Ca（OH）2相比具有显着的OPN免疫标记作用。仅BS-2P糊剂显示出与Ca（OH）2相当的抗菌活性。与对照组和Ca（OH）2组相比，F18组具有显着更多的OPN免疫标记（P <0.05）。所有糊剂均减少粪肠球菌的总数；但是，仅当将BS-2P和Ca（OH）2组与对照组进行比较时，这种减少才有意义（P <0.05）。仅氢氧化钙消除了粪肠球菌。结论实验性BS-2P和F18糊剂与Ca（OH）2相比具有生物相容性，可刺激生物矿化并诱导显着的OPN免疫标记。仅BS-2P糊剂显示出与Ca（OH）2相当的抗菌活性。与对照组和Ca（OH）2组相比，F18组的OPN免疫标记明显更多（P <0.05）。所有糊剂均减少粪肠球菌的总数；然而，只有将BS-2P组和Ca（OH）2组与对照组进行比较时，这种减少才有意义（P <0.05）。仅氢氧化钙消除了粪肠球菌。结论实验性BS-2P和F18糊剂与Ca（OH）2相比具有生物相容性，可刺激生物矿化并诱导显着的OPN免疫标记。仅BS-2P糊剂显示出与Ca（OH）2相当的抗菌活性。结论实验性BS-2P和F18糊剂具有生物相容性，可刺激生物矿化并与Ca（OH）2相比具有显着的OPN免疫标记作用。仅BS-2P糊剂显示出与Ca（OH）2相当的抗菌活性。结论实验性BS-2P和F18糊剂具有生物相容性，可刺激生物矿化并与Ca（OH）2相比具有显着的OPN免疫标记作用。仅BS-2P糊剂显示出与Ca（OH）2相当的抗菌活性。