Liver diseases are one of the leading causes of mortality in the world, mainly caused by different etiological agents, alcohol consumption, viruses, drug intoxication, and malnutrition. The maturation of gene therapy has heralded new avenues for developing effective interventions for these diseases. Derived from a remarkable microbial defense system, clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins 9 system (CRISPR/Cas9 system) is driving innovative applications from basic biology to biotechnology and medicine. Recently, the mutagenic function of CRISPR/Cas9 system has been widely adopted for genome and disease research. In this review, we describe the development and applications of CRISPR/Cas9 system on liver diseases for research or translational applications, while highlighting challenges as well as future avenues for innovation.

中文翻译：

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肝脏疾病中的 CRISPR/Cas9 相关技术：从可行性到未来的多样性。

肝病是世界上导致死亡的主要原因之一，主要由不同的病原体、饮酒、病毒、药物中毒和营养不良引起。基因疗法的成熟预示着为这些疾病开发有效干预措施的新途径。源自卓越的微生物防御系统，成簇的规则间隔短回文重复序列/CRISPR 相关蛋白 9 系统（CRISPR/Cas9 系统）正在推动从基础生物学到生物技术和医学的创新应用。最近，CRISPR/Cas9 系统的致突变功能已被广泛用于基因组和疾病研究。在这篇综述中，我们描述了 CRISPR/Cas9 系统在肝病研究或转化应用中的发展和应用，