Abstract

Background

SIRT1 and HDAC 9 genes are related to inflammation and may contribute to the pathogenesis of coronary artery disease (CAD). We aimed to evaluate the expression level, methylation profile and polymorphisms of these genes in CAD patients.

Methods

In this study, 50 CAD patients and 50 healthy individuals were recruited. The expression level change was evaluated using the TaqMan Real-Time PCR method. The methylation of genes promoter and genotyping of polymorphisms were evaluated by the HRM.

Results

The expression level of SIRT1 was reduced while the HDAC9 expression level showed a significant elevation (p < .001). The SIRT1 gene promoter was hypomethylated and the HDAC9 gene promoter was hypermethylated in CAD patients. Also, CG + GG genotype in SIRT1 and both genotypes in the HDAC9 gene were associated with expression change.

Conclusions

SIRT1 and HDAC9 genes, expression changes can be suggested as a potential biomarker for CAD detection.

中文翻译：

---

冠状动脉疾病中 sirtuin-1 和组蛋白脱乙酰酶 9 的反向表达模式

摘要

背景

SIRT1和HDAC 9 基因与炎症有关，可能与冠状动脉疾病 (CAD) 的发病有关。我们旨在评估这些基因在 CAD 患者中的表达水平、甲基化谱和多态性。

方法

在这项研究中，招募了 50 名 CAD 患者和 50 名健康人。使用 TaqMan 实时 PCR 方法评估表达水平变化。通过 HRM 评估基因启动子的甲基化和多态性的基因分型。

结果

SIRT1的表达水平降低，而HDAC9 的表达水平显着升高 ( p  < .001)。在 CAD 患者中，SIRT1 基因启动子被低甲基化，HDAC9 基因启动子被高甲基化。此外， SIRT1中的 CG + GG 基因型和HDAC9基因中的两种基因型都与表达变化相关。

结论

SIRT1和HDAC9基因的表达变化可作为 CAD 检测的潜在生物标志物。