This study was conducted in order to understand key biochemical, physiological and molecular changes associated with ovarian growth and with lipid transfer and/or accumulation into the ovary during oogenesis in captive beluga sturgeon. Plasma levels of triacylglycerides, cholesterol, phospholipid and sex steroid hormones were determined and found to all increase notably throughout development from the perinucleolar to the tertiary yolk stage. Using fast protein liquid chromatography, three major lipoprotein peaks were recognized in chromatograms from all samples. These peaks were characterised as containing very low density lipoprotein (Vldl), low density lipoprotein/ high density lipoprotein (Ldl/Hdl) and plasma proteins, respectively. While Ldl/Hdl represented the most abundant lipoprotein fraction, relative abundance of different lipoprotein classes did not change with stage of oogenesis. Eluted lipoproteins were separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis and sequenced. The peptide sequence spectra for 66, 205, 29 and 70 kDa bands matched with albumin, vitellogenin (Vtg) AB2b, immunoglobulin light-chain precursor and immunoglobulin heavy-chain, respectively. The large amount of albumin in the plasma protein peak and the presence of Vtg AB2b to within Ldl/Hdl reinforce the lipoprotein classification. Lastly, transcript levels of ovarian lipoprotein lipase (lpl), apolipoprotein E (apoe) very low density lipoprotein receptors (vldlr), and low-density lipoprotein receptor-related protein 8-like (lrp8) were estimated using quantitative polymerase chain reaction. The high mRNA levels of lpl, apoe, and lipoprotein receptors vldlr and lrp8, in previtellogenic females suggest that sturgeon oocytes need to be prepared to accept and traffic Vtg and lipids internally, prior to the start of vitellogenesis.

中文翻译：

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养殖的白lu鱼卵巢发育过程中脂质生物学的变化

进行这项研究的目的是了解圈养的白lu的卵生过程中与卵巢生长以及脂质转移和/或积累到卵巢中有关的关键生化，生理和分子变化。测定血浆三酰甘油酯，胆固醇，磷脂和性类固醇激素的水平，发现它们在从核仁到卵黄期的整个发育过程中均显着增加。使用快速蛋白质液相色谱法，在所有样品的色谱图中识别出三个主要的脂蛋白峰。这些峰的特征是分别含有极低密度脂蛋白（Vldl），低密度脂蛋白/高密度脂蛋白（Ldl / Hdl）和血浆蛋白。虽然Ldl / Hdl代表最丰富的脂蛋白部分，不同脂蛋白类别的相对丰度并没有随着卵子发生的阶段而改变。使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳分离洗脱的脂蛋白并测序。分别与白蛋白，卵黄蛋白原（Vtg）AB2b，免疫球蛋白轻链前体和免疫球蛋白重链匹配的66、205、29和70 kDa条带的肽序列光谱。血浆蛋白峰中大量白蛋白和Ldl / Hdl中Vtg AB2b的存在​​增强了脂蛋白的分类。最后，使用定量聚合酶链反应评估了卵巢脂蛋白脂肪酶（lpl），载脂蛋白E（apoe）极低密度脂蛋白受体（vldlr）和低密度脂蛋白受体相关蛋白8-like（lrp8）的转录水平。Lpl，Apoe，