Many human diseases, such as obesity and diabetes, show annual increases in prevalence and often involve intestinal microbes. One such probiotic bacterium, Akkermansia muciniphila, which was discovered a decade ago, has been reported to influence glucose homeostasis and to contribute to gut health. Amuc_1100, a functionally uncharacterized protein of A. muciniphila, was found to be a key active component in reducing the body weight of mice. Here, the crystal structure of Amuc_1100 (residues 31–317), referred to as Amuc_1100*, is reported at 2.1 Å resolution. Amuc_1100* has a similar fold to three proteins related to pilus formation, PilO, PilN and EpsL, indicating a similar function. Biochemical investigations further confirmed a monomeric state for the soluble region of Amuc_1100, which differs from the dimeric states of PilO, PilN and EpsL. This study provides a structural basis for the elucidation of the molecular mechanism of Amuc_1100.

中文翻译：

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得自Akkermansia muciniphila的单体Amuc_1100的晶体结构。

许多人类疾病，例如肥胖症和糖尿病，显示患病率逐年增加，并经常涉及肠道微生物。十年前就发现了一种这样的益生菌细菌Akkermansia muciniphila，它会影响葡萄糖体内稳态并有助于肠道健康。Amuc_1100，黏液曲霉的功能未知蛋白被发现是降低小鼠体重的关键活性成分。在这里，Amuc_1100（残基31-317）的晶体结构被称为Amuc_1100 *，其分辨率为2.1。Amuc_1100 *具有与菌毛形成相关的三种蛋白质PilO，PilN和EpsL相似的折叠，表明其功能相似。生化研究进一步证实了Amuc_1100可溶性区域的单体状态，该状态不同于PilO，PilN和EpsL的二聚状态。该研究为阐明Amuc_1100的分子机制提供了结构基础。