Contemporary sequencing technologies, Oxford Nanopore in particular, provide a way to sequence multiple samples during single run using molecular barcodes. Specific circumstances, however, can make barcoding undesirable or unaffordable. Here, we introduce Barapost: a command-line toolkit that demultiplexes long nucleotide sequences relying on taxonomic annotation instead of barcoding.

中文翻译：

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Barapost：根据分类注释对核苷酸序列进行分箱

现代测序技术，尤其是 Oxford Nanopore，提供了一种在单次运行期间使用分子条形码对多个样品进行测序的方法。但是，特定情况可能会使条形码变得不受欢迎或负担不起。在这里，我们介绍 Barapost：一个命令行工具包，它依赖于分类注释而不是条形码来解复用长核苷酸序列。