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Cell-free supernatant of Streptococcus salivarius M18 impairs the pathogenic properties of Pseudomonas aeruginosa and Klebsiella pneumonia
Archives of Microbiology ( IF 2.8 ) Pub Date : 2020-08-03 , DOI: 10.1007/s00203-020-02005-8
Sinem Tunçer 1, 2 , Sevinç Karaçam 2, 3
Affiliation  

M18 strain of Streptococcus salivarius is a bacterial replacement probiotic that has been suggested for use in the oral cavity. Here, we have shown that S. salivarius M18 cell-free supernatant reduced the growth of the two most common human pathogens Pseudomonas aeruginosa and Klebsiella pneumonia and sensitized the pathogenic bacteria to antibiotic. Besides, the supernatant inhibited biofilm formation of P. aeruginosa drastically. For pinpointing the biomolecular changes that occurred in P. aeruginosa incubated with the probiotic supernatant, attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy was used. Unsupervised learning algorithms, principal component analysis (PCA) and hierarchical cluster analysis (HCA), and intensity analyses of individual spectral bands exhibited comprehensive alterations in the polysaccharide and lipid contents and compositions of P. aeruginosa cultivated with S. salivarius M18 cell-free supernatant. These results indicate that S. salivarius M18 has the potential for the prevention or alleviation of different pathogen-induced infections along with the infections of oral pathogens.

中文翻译:

唾液链球菌 M18 的无细胞上清液损害铜绿假单胞菌和肺炎克雷伯菌的致病特性

唾液链球菌 M18 菌株是一种细菌替代益生菌,已建议用于口腔。在这里,我们已经表明,唾液链球菌 M18 无细胞上清液减少了两种最常见的人类病原体铜绿假单胞菌和肺炎克雷伯菌的生长,并使病原菌对抗生素敏感。此外,上清液显着抑制了铜绿假单胞菌的生物膜形成。为了查明与益生菌上清液一起孵育的铜绿假单胞菌中发生的生物分子变化,使用了衰减全反射傅里叶变换红外 (ATR-FTIR) 光谱。无监督学习算法,主成分分析(PCA)和层次聚类分析(HCA),单个光谱带的强度和强度分析显示,用唾液链球菌 M18 无细胞上清液培养的铜绿假单胞菌的多糖和脂质含量和组成发生了全面变化。这些结果表明,唾液链球菌 M18 具有预防或减轻不同病原体引起的感染以及口腔病原体感染的潜力。
更新日期:2020-08-03
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