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Polyhydroxyalkanoate synthesis by bacteria isolated from landfill and ETP with pomegranate peels as carbon source
Archives of Microbiology ( IF 2.8 ) Pub Date : 2020-08-03 , DOI: 10.1007/s00203-020-01995-9
Vijay Rayasam 1 , Pooja Chavan 2 , Tarika Kumar 3
Affiliation  

Many bacteria produce polyhydroxyalkanoates (PHAs) when exposed to stressful conditions. It is a known fact that PHAs have the potential to replace petrochemical-based plastics as they are biodegradable, biocompatible, and thermoprocessible materials. The study focusses on producing PHA from microbes isolated from polluted environments and pomegranate peels were utilized as a unique carbon source. This was done to ensure reduction in the cost of the substrate that has not yet been reported as a substrate for PHA production. A comparative study was also done with Cupriavidus necator, the reference strain. Out of many bacterial strains, isolated, eight of these were found to have ability to produce PHA. Pomegranate peel was substituted as carbon source in the medium and all bacterial isolates along with reference strain were used to test their ability to produce PHA from waste. Cupriavidus necator, the reference strain, yielded 71% PHA. Bacillus halotolerans DSM8802 yielded 83% at 1:1:: C:N ratio at 72 h.

中文翻译:

以石榴皮为碳源,从垃圾填埋场和 ETP 中分离的细菌合成聚羟基链烷酸酯

当暴露于压力条件下时,许多细菌会产生聚羟基链烷酸酯 (PHA)。众所周知,PHA 具有取代石化塑料的潜力,因为它们是可生物降解、生物相容性和可热加工的材料。该研究的重点是从污染环境中分离出的微生物生产 PHA,石榴皮被用作独特的碳源。这样做是为了确保降低尚未作为 PHA 生产底物报道的底物的成本。还对参考菌株 Cupriavidus necator 进行了比较研究。在分离的许多细菌菌株中,发现其中的八种具有产生 PHA 的能力。石榴皮被替换为培养基中的碳源,所有细菌分离物和参考菌株都被用来测试它们从废物中产生 PHA 的能力。Cupriavidus necator,参考菌株,产生71%的PHA。耐盐芽孢杆菌 DSM8802 在 1:1::C:N 比例下在 72 小时的产率为 83%。
更新日期:2020-08-03
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