OBJECTIVE To know the potential role of IKKα (an NF-κB noncanonical pathway) in gastric precancerous lesion via mediating Maspin. METHODS Gastric cancer, precancerous lesion and control tissues (chronic non-atrophic gastritis) were collected for determining the expression of IKKα and Maspin by immunohistochemistry. Thereafter, gastric precancerous models were established and divided into the Control group, Model group and Model + shIKKα group. All rats were subjected to observe the pathological changes and ultramicro structure of the gastric mucosa by HE staining or electron microscope, and to measure the serum levels of inflammatory cytokines by ELISA, the expression of apoptosis-related proteins by immunohistochemistry, as well as the expression of IKKα and Maspin by quantitative real-time PCR and Western blotting. RESULTS Precancerous lesion and gastric cancer tissues manifested significant upregulation of IKKα positive expression, concomitant with downregulation of the positive expression of Maspin, and these changes were more evident in the gastric cancer tissues. In comparison with the Control group, rats in the Model group had significant increases in serum levels of TNF-α, IL-1β, IL-6 and COX-2, with up-regulations of Bcl-2, CyclinD1, IKKα and p-IKKα, and down-regulations of Bax, Caspase-3 and Maspin. shIKKα treatment attenuate inflammation and apoptosis in gastric precancerous lesion (GPL) rat, with the downregulation of IKKα and p-IKKα, and upregulation of Maspin. CONCLUSION Inhibiting IKKα, via upregulating Maspin, can mitigate the inflammation and promote cell apoptosis in precancerous rats, thereby delaying the development of the precancerous lesions.

中文翻译：

---

通过介导Maspin，IKKα在胃癌前病变中的潜在功能。

目的通过介导Maspin了解IKKα（一种NF-κB非典型途径）在胃癌前病变中的潜在作用。方法收集胃癌，癌前病变和对照组织（慢性非萎缩性胃炎），采用免疫组织化学方法检测IKKα和Maspin的表达。此后，建立胃癌前期模型并分为对照组，模型组和模型+shIKKα组。用HE染色或电镜观察大鼠胃黏膜的病理变化和超微结构，用ELISA法测定血清中炎性细胞因子的水平，用免疫组织化学方法检测细胞凋亡相关蛋白的表达及表达。实时定量PCR和Western印迹检测IKKα和Maspin。结果癌前病变和胃癌组织表现出IKKα阳性表达的显着上调，并伴随Maspin阳性表达的下调，这些变化在胃癌组织中更为明显。与对照组相比，模型组大鼠的血清TNF-α，IL-1β，IL-6和COX-2水平明显升高，而Bcl-2，CyclinD1，IKKα和p- IKKα，以及Bax，Caspase-3和Maspin的下调。shIKKα治疗可减轻胃癌前病变（GPL）大鼠的炎症和细胞凋亡，同时下调IKKα和p-IKKα的表达，并上调Maspin的表达。结论通过上调Maspin抑制IKKα可减轻癌前期大鼠的炎症反应并促进细胞凋亡，