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Cancer cell migration and cancer drug screening in oxygen tension gradient chip.
Biomicrofluidics ( IF 3.2 ) Pub Date : 2020-07-21 , DOI: 10.1063/5.0011216
Hyeono Nam 1 , Kenichi Funamoto , Jessie S Jeon
Affiliation  

Cancer metastasis, which is prevalent in malignant tumors, is present in a variety of cases depending on the primary tumor and metastatic site. The cancer metastasis is affected by various factors that surround and constitute a tumor microenvironment. One of the several factors, oxygen tension, can affect cancer cells and induce changes in many ways, including motility, directionality, and viability. In particular, the oxygen tension gradient is formed within a tumor cluster and oxygen is lower toward the center of the cluster from the perivascular area. The simple and efficient designing of the tumor microenvironment using microfluidic devices enables the simplified and robust platform of the complex in vivo microenvironment while observing a clear cause-and-effect between the properties of cancer cells under oxygen tension. Here, a microfluidic device with five channels including a gel channel, media channels, and gas channels is designed. MDA-MB-231cells are seeded in the microfluidic device with hydrogel to simulate their three-dimensional movement in the body. The motility and directionality of the cancer cells under the normoxic and oxygen tension gradient conditions are compared. Also, the viability of the cancer cells is analyzed for each condition when anticancer drugs are applied. Unlike the normoxic condition, under the oxygen tension gradient, cancer cells showed directionality toward higher oxygen tension and decreased viability against the certain anticancer drug. The simplified design of the tumor microenvironment through microfluidic devices enables comprehension of the response of cancer cells to varying oxygen tensions and cancer drugs in the hypoxic tumor microenvironment.

中文翻译:

氧气张力梯度芯片中的癌细胞迁移和癌症药物筛选。

取决于原发肿瘤和转移部位,在各种情况下都存在恶性肿瘤中普遍存在的癌症转移。癌转移受围绕并构成肿瘤微环境的各种因素影响。氧气压力是多种因素之一,可以影响癌细胞并以多种方式诱导变化,包括运动性,方向性和生存力。特别地,在肿瘤簇内形成氧张力梯度,并且从血管周围区域朝向簇的中心降低氧。使用微流控设备对肿瘤微环境进行简单而有效的设计可以简化体内复杂的平台微环境,同时在氧气压力下观察癌细胞特性之间的明确因果关系。在此,设计了具有五个通道的微流体装置,所述五个通道包括凝胶通道,介质通道和气体通道。将MDA-MB-231细胞植入带有水凝胶的微流体装置中,以模拟它们在体内的三维运动。比较了常氧和氧张力梯度条件下癌细胞的运动性和方向性。同样,当应用抗癌药物时,针对每种情况分析癌细胞的生存力。与常氧条件不同,在氧张力梯度下,癌细胞表现出朝向较高氧张力的方向性,并且对某些抗癌药的生存力降低。
更新日期:2020-09-01
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