Rapid and frequent screening of cytokines as immunomodulation agents is necessary for precise interventions in severe pathophysiological conditions. In addition to high-sensitivity detection of such analytes in complex biological fluids such as blood, saliva, and cell culture medium samples, it is also crucial to work out miniaturized bioanalytical platforms with potential for high-density integration enabling screening of multiple analytes. In this work, we show a compact, point-of-care-ready bioanalytical platform for screening of cytokines such as interleukin-4 (IL-4) and interleukin-2 (IL-2) based on one-dimensional ion-sensitive field-effect transistors arrays (nanoISFETs) of silicon fabricated at wafer-scale via nanoimprint lithography. The nanoISFETs biofunctionalized with receptor proteins alpha IL-4 and alpha IL-2 were deployed for screening cytokine secretion in mouse T helper cell differentiation culture media, respectively. Our nanoISFETs showed robust sensor signals for specific molecular binding and can be readily deployed for real-time screening of cytokines. Quantitative analyses of the nanoISFET-based bioanalytical platform was carried out for IL-4 concentrations ranging from 25 fg/mL (1.92 fM) to 2.5 μg/mL (192 nM), showing a limit of detection down to 3–5 fM, which was found to be in agreement with ELISA results in determining IL-4 concentrations directly in complex cell culture media.

快速和频繁地筛选作为免疫调节剂的细胞因子对于在严重的病理生理条件下进行精确干预是必要的。除了对复杂生物流体（如血液、唾液和细胞培养基样品）中的此类分析物进行高灵敏度检测外，开发具有高密度集成潜力的微型生物分析平台也至关重要，可以筛选多种分析物。在这项工作中，我们展示了一个紧凑的、即时可用的生物分析平台，用于基于一维离子敏感场筛选细胞因子，如白细胞介素 4 (IL-4) 和白细胞介素 2 (IL-2)通过纳米压印光刻在晶圆级制造的硅效应晶体管阵列（nanoISFET）。用受体蛋白 α IL-4 和 α IL-2 生物功能化的 nanoISFET 分别用于筛选小鼠 T 辅助细胞分化培养基中的细胞因子分泌。我们的 nanoISFET 显示出针对特定分子结合的强大传感器信号，并且可以轻松部署用于细胞因子的实时筛选。对基于 nanoISFET 的生物分析平台进行了定量分析，IL-4 的浓度范围从 25 fg/mL (1.92 fM) 到 2.5 μg/mL (192 nM)，显示检测限低至 3-5 fM，其中发现与直接在复杂细胞培养基中测定 IL-4 浓度的 ELISA 结果一致。我们的 nanoISFET 显示出针对特定分子结合的强大传感器信号，并且可以轻松部署用于细胞因子的实时筛选。对基于 nanoISFET 的生物分析平台进行了定量分析，IL-4 的浓度范围从 25 fg/mL (1.92 fM) 到 2.5 μg/mL (192 nM)，显示检测限低至 3-5 fM，其中发现与直接在复杂细胞培养基中测定 IL-4 浓度的 ELISA 结果一致。我们的 nanoISFET 显示出针对特定分子结合的强大传感器信号，并且可以轻松部署用于细胞因子的实时筛选。对基于 nanoISFET 的生物分析平台进行了定量分析，IL-4 的浓度范围从 25 fg/mL (1.92 fM) 到 2.5 μg/mL (192 nM)，显示检测限低至 3-5 fM，其中发现与直接在复杂细胞培养基中测定 IL-4 浓度的 ELISA 结果一致。