This study describes the community composition and functions of the gut microbiome of the freshwater omnivorous pufferfish based on metagenomic approach. Metagenome sequence data showed a dominance of the class Gammaproteobacteria followed by Fusobacteria, Actinobacteria, Anerolineae, Betaproteobacteria, Deinococci, Clostridia and Deltaproteobacteria. At the order level, the most abundant groups were Aeromonadales, Fusobacteriales, Enterobacterales, Synechococcales. The genus Aeromonas was the most predominant followed by Plesiomonas and Cetobacterium. Additionally, within the domain Archaea, class Methanomicrobia was most abundant followed by Hadesarchaea, Thermoplasmata, Candidatus Altiarchaeales, Candidatus Bathyarchaeota and Thermoprotei. The metabolic profile of the bacterial community exhibited a high prevalence of genes associated with core housekeeping functions, such as synthesis of cofactors, vitamins, prosthetic groups, pigments, amino acids and its derivatives, carbohydrate and protein metabolism. Comparative analysis with other fish gut microbiome showing similarity in protein metabolism with carnivorous Salmon and carbohydrate metabolism with herbivorous grass carp respectively. This study describes the bacterial community compositions are influenced by the trophic level.

中文翻译：

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淡水河豚（Tetraodon cutcutia）肠道微生物组的组成和功能表征

本研究基于宏基因组学方法描述了淡水杂食性河豚的肠道微生物群落组成和功能。宏基因组序列数据显示 Gammaproteobacteria 类占主导地位，其次是 Fusobacteria、Actinobacteria、Anerolineae、Betaproteobacteria、Deinococci、Clostridia 和 Deltaproteobacteria。在订单级别，最丰富的群体是气单胞菌目、梭杆菌目、肠杆菌目、聚球藻目。气单胞菌属是最主要的，其次是 Plesiomonas 和 Cetobacterium。此外，在古细菌领域内，甲烷微生物类最丰富，其次是 Hadesarchaea、Thermoplasmata、Candidatus Altiarchaeales、Candidatus Bathyarchaeota 和 Thermoprotei。细菌群落的代谢特征表现出与核心管家功能相关的基因的高度流行，例如辅助因子、维生素、假体基团、色素、氨基酸及其衍生物、碳水化合物和蛋白质代谢的合成。与其他鱼肠道微生物组的比较分析显示，蛋白质代谢与食肉鲑鱼和碳水化合物代谢分别与食草草鱼相似。这项研究描述了细菌群落组成受营养水平的影响。与其他鱼肠道微生物组的比较分析显示，蛋白质代谢与食肉鲑鱼和碳水化合物代谢分别与食草草鱼相似。这项研究描述了细菌群落组成受营养水平的影响。与其他鱼肠道微生物组的比较分析显示，蛋白质代谢与食肉鲑鱼和碳水化合物代谢分别与食草草鱼相似。这项研究描述了细菌群落组成受营养水平的影响。