Chia (Salvia hispanica) seed peptides have drawn attention because of their antioxidant, antihypertensive and anti-inflammatory activities, making them ideal candidates for development of cosmeceutical skin products. However, there are no preceding reports that address their aging-related enzyme inhibitory activities. The aim of this study was to investigate the in vitro and in silico inhibitory activity of chia seed peptides towards the main aging-related enzymes. Enzyme-inhibition activity of < 3 kDa chia seed peptides towards collagenase, hyaluronidase, tyrosinase, and elastase was evaluated. Further fractions were obtained by size exclusion chromatography (SEC) and re-tested for enzyme inhibitory activity. Peptide sequences were identified from the most effective fraction and used for in silico analysis. The < 3 kDa peptides exhibited inhibitory activities towards elastase (65.32%, IC₅₀ = 0.43 mg/mL), tyrosinase (58.74%, IC₅₀ = 0.66 mg/mL), hyaluronidase (26.96%, IC₅₀ = 1.28 mg/mL), and collagenase (28.90%, IC₅₀ = 1.41 mg/mL). They showed mixed-type inhibition patterns towards elastase and hyaluronidase, while a non-competitive inhibition pattern was observed towards collagenase and tyrosinase. Fraction II obtained by SEC, showed higher enzyme inhibitory activity. Seven peptides were identified in this fraction (APHWYTN, DQNPRSF, GDAHWAY, GDAHWTY, GDAHWVY, GFEWITF, and KKLKRVYV), which according to in silico analysis, possess 19–29 enzyme–peptide pair interactions towards elastase and three peptide sequences shared homology sequence (GDAHW). These results demonstrate that peptides from chia seeds may contribute in the improvement of skin health by offering protection against aging-related enzymes by preventing degradation of the protein matrix on the skin; however, further in vivo studies are needed to evaluate its actual capability.

Chia（西班牙丹参）种子肽因其抗氧化，降压和抗炎作用而备受关注，使其成为药妆护肤产品开发的理想之选。但是，以前没有报道涉及其衰老相关的酶抑制活性。这项研究的目的是研究正大种子多肽对主要衰老相关酶的体外和计算机抑制活性。评估了<3 kDa奇亚籽肽对胶原酶，透明质酸酶，酪氨酸酶和弹性蛋白酶的酶抑制活性。通过大小排阻色谱法（SEC）获得更多的级分，并重新测试酶抑制活性。从最有效的馏分中鉴定出肽序列，并将其用于计算机分析。<₅₀  = 0.43 mg / mL），酪氨酸酶（58.74％，IC ₅₀  = 0.66 mg / mL），透明质酸酶（26.96％，IC ₅₀  = 1.28 mg / mL）和胶原酶（28.90％，IC ₅₀ = 1.41 mg / mL）。他们表现出对弹性蛋白酶和透明质酸酶的混合型抑制模式，而对胶原酶和酪氨酸酶的抑制性非竞争性模式。通过SEC获得的级分II显示出更高的酶抑制活性。在该级分中鉴定出七个肽段（APHWYTN，DQNPRSF，GDAHWAY，GDAHWTY，GDAHWVY，GFEWITF和KKLKRVYV），根据计算机分析，它们与弹性蛋白酶具有19-29个酶-肽对相互作用，并且三个肽段共有同源序列（ GDAHW）。这些结果表明，来自奇亚籽的肽可通过防止皮肤上蛋白质基质的降解，提供针对衰老相关酶的保护，从而有助于改善皮肤健康。然而，需要进一步的体内研究来评估其实际能力。