Sapoviruses are increasingly being recognized as pathogens associated with gastroenteritis in humans. Human sapoviruses are currently assigned to 18 genotypes (GI.1-7, GII.1-8, GIV.1, and GV.1-2) based on the sequence of the region encoding the major structural protein. In this study, we evaluated 11 polymerase chain reaction (PCR) assays using published and newly designed/modified primers and showed that four PCR assays with different primer combinations amplified all of the tested human sapovirus genotypes using either synthetic DNA or cDNA prepared from human sapovirus-positive fecal specimens. These assays can be used as improved broadly reactive screening tests or as tools for molecular characterization of human sapoviruses.

沙波病毒被越来越多地认为是与人类胃肠炎有关的病原体。根据编码主要结构蛋白的区域的顺序，目前人类沙波病毒被分配为18种基因型（GI.1-7，GII.1-8，GIV.1和GV.1-2）。在这项研究中，我们评估了11种聚合酶链反应（PCR）测定法，使用已发布和新设计/修饰的引物，结果显示，四种具有不同引物组合的PCR测定法使用从人血球蛋白制备的合成DNA或cDNA扩增了所有测试的人血球病毒基因型粪便阳性。这些测定法可用作改进的广泛反应性筛选试验或用作人沙波病毒分子表征的工具。