In the present research, field‐amplified sample injection–CZE (FASI–CZE) coupled with a diode array detector was established to determine trace level sulfa antibiotic. Sulfathiazole, sulfadiazine, sulfamethazine, sulfadimethoxine, sulfamethoxazole, and sulfisoxazole were selected as analytes for the experiments. The background electrolyte solution consisted of 70.0 mmol/L borax and 60.0 mmol/L boric acid (including 10% methanol, pH 9.1). The plug was 2.5 mmol/L borax, which was injected into the capillary at a pressure of 0.5 psi for 5 s. Then the sample was injected into the capillary at an injection voltage of –10 kV for 20 s. The electrophoretic separation was carried out under a voltage of +19 kV. The capillary temperature was maintained at 20˚C throughout the analysis, and six sulfonamides were completely separated within 35 min. Compared with pressure injection‐CZE, the sensitivity of FASI‐CZE was increased by 6.25–10.0 times, and the LODs were reduced from 0.2–0.5 to 0.02–0.05 μg/mL. The method was applied to the determination of sulfonamides in river water and particulate matter samples. The recoveries were 78.59–106.59%. The intraday and interday precisions were 2.89–7.35% and 2.77–7.09%, respectively. This provides a simpler and faster method for the analysis of sulfa antibiotic residues in environmental samples.

中文翻译：

---

通过现场放大的样品注入毛细管区带电泳法测定河流和颗粒物中的磺胺类抗生素残留。

在本研究中，建立了带有二极管阵列检测器的现场放大样品进样CZE（FASI-CZE），以确定痕量磺胺类抗生素。选择磺胺噻唑，磺胺嘧啶，磺胺二甲嘧啶，磺胺二甲恶嗪，磺胺甲恶唑和磺胺异恶唑作为实验的分析物。背景电解质溶液由70.0 mmol / L硼砂和60.0 mmol / L硼酸（包括10％的甲醇，pH 9.1）组成。塞子为2.5 mmol / L硼砂，将其以0.5 psi的压力注入毛细管5 s。然后将样品以-10 kV的注入电压注入毛细管中20 s。电泳分离在+ 19kV的电压下进行。在整个分析过程中，毛细管温度保持在20°C，并且在35分钟内将六种磺酰胺完全分离。与压力注入CZE相比，FASI-CZE的灵敏度提高了6.25-10.0倍，LOD从0.2-0.5降低到0.02-0.05μg/ mL。该方法用于测定河水和颗粒物样品中的磺胺类药物。回收率为78.59-106.59％。日内和日间精度分别为2.89-7.35％和2.77-7.09％。这为分析环境样品中的磺胺类抗生素残留提供了一种更简单，更快速的方法。分别。这为分析环境样品中的磺胺类抗生素残留提供了一种更简单，更快速的方法。分别。这为分析环境样品中的磺胺类抗生素残留提供了一种更简单，更快速的方法。