The role of plasmacytoid dendritic cells (pDCs), and myeloid dendritic cells (mDCs) in women with preeclampsia has not been elucidated. We compared the frequency of peripheral pDCs, mDCs, NK cells, and T helper 17 (Th17) cells among non-pregnant/pregnant women, and women with early-/late-onset preeclampsia. We examined pDCs and mDCs using Anti-Human Lineage Cocktail 3 (CD3, CD14, CD19, and CD20), HLA-DR, CD11c, and CD123. We detected NK cells using Lineage cocktail, CD8, CD16, and CD56. We determined Th17 cells using CD3, CD4, CD8, CXCR3, and CCR6. We recruited 13 non-pregnant women, 50 normal pregnant women, 13 women with early-onset preeclampsia (onset at <34 gestational weeks), and 10 women with late-onset preeclampsia. The fraction of pDCs in women with early-onset preeclampsia was significantly lower than in non-pregnant women and normal pregnant women at 19-29 gestational weeks (4.1 % vs. 41.2 % and 19.0 %, respectively [p = 0.0005, and p = 0.025]), however, the fraction of pDCs in late-onset preeclampsia was not significantly different from normal pregnant women at 37 gestational weeks (11.1 % vs. 29.1 %, respectively [p = 0.149]), although it was significantly lower than in non-pregnant women (11.1 % vs. 41.2 %, respectively [p = 0.044]). The fraction of Th17 cells in women with early-onset preeclampsia was significantly higher than in normal pregnant women at 19-29 gestational weeks (p = 0.022). In conclusion, the level of circulating pDCs was lower in early-onset preeclampsia than in non-pregnant and pregnant women, suggesting the role of pDCs in the pathogenesis of early-onset preeclampsia.

中文翻译：

---

降低早发性先兆子痫妇女中浆细胞样树突细胞的循环水平。

尚未阐明浆细胞样树突细胞 (pDC) 和髓样树突细胞 (mDC) 在先兆子痫女性中的作用。我们比较了非孕妇/孕妇和早发性/晚发性先兆子痫妇女的外周 pDC、mDC、NK 细胞和 T 辅助 17 (Th17) 细胞的频率。我们使用 Anti-Human Lineage Cocktail 3（CD3、CD14、CD19 和 CD20）、HLA-DR、CD11c 和 CD123 检查了 pDC 和 mDC。我们使用谱系鸡尾酒、CD8、CD16 和 CD56 检测了 NK 细胞。我们使用 CD3、CD4、CD8、CXCR3 和 CCR6 确定了 Th17 细胞。我们招募了 13 名非孕妇、50 名正常孕妇、13 名早发性先兆子痫（<34 孕周发病）和 10 名迟发性子痫前期妇女。在妊娠 19-29 周时，早发性先兆子痫妇女的 pDC 比例显着低于非孕妇和正常孕妇（分别为 4.1 % 与 41.2 % 和 19.0 % [p = 0.0005，p = 0.025])，然而，迟发性先兆子痫中 pDC 的比例在 37 孕周时与正常孕妇没有显着差异（分别为 11.1 % 和 29.1 % [p = 0.149]），尽管它显着低于非孕妇（分别为 11.1 % 和 41.2 % [p = 0.044]）。早发性先兆子痫妇女的 Th17 细胞比例在 19-29 孕周时显着高于正常孕妇（p = 0.022）。总之，早发性先兆子痫的循环 pDCs 水平低于非孕妇和孕妇，