This study aimed to determine whether agar dilution, research-use-only disk diffusion (Mast Group Ltd., Bootle Merseyside, UK), Etest (bioMérieux, Inc., Durham, NC), and MIC test strip (MTS) (Liofilchem, Inc., Waltham, MA) methods yield equivalent results to those of broth microdilution (BMD) for imipenem-relebactam susceptibility testing using a collection of 297 Gram-negative bacilli, including members of the order Enterobacterales and Pseudomonas aeruginosa, enriched for drug resistance. MIC and disk diameter results were interpreted using United States Food and Drug Administration breakpoints. Overall, 76.8% of the isolates tested were susceptible to imipenem-relebactam by BMD. MIC values for agar dilution, Etest, and MTS were not significantly different from that for BMD, although they tended to be 1 to 2 dilutions higher. Essential agreement was 95.6% for agar dilution, 90.6% for Etest, and 85.2% for MTS. Categorical agreement was 98.0% for agar dilution, 73.1% for disk diffusion, 96.3% for Etest, and 96.6% for MTS. In conclusion, agar dilution and Etest yielded comparable results to BMD for imipenem-relebactam.

中文翻译：

---

琼脂稀释，圆盘扩散和梯度剥离法与肉汤微量稀释相比，亚胺培南-雷巴坦对革兰氏阴性杆菌的药敏试验。

这项研究的目的是确定琼脂稀释液，仅用于研究的圆盘扩散法（Mast Group Ltd.，布特尔默西赛德郡，英国），Etest（bioMérieux，Inc.，达勒姆，北卡罗来纳州）和MIC测试条（MTS）（Liofilchem， Inc.，Waltham，MA）的方法产生的结果与使用297革兰氏阴性杆菌（包括肠杆菌和铜绿假单胞菌成员）进行亚胺培南-雷巴坦敏感性测试的肉汤微量稀释（BMD）的结果相同，丰富了抗药性。MIC和圆盘直径结果使用美国食品和药物管理局的断点进行解释。总体而言，BMD对76.8％的分离株易感亚胺培南-雷巴坦。琼脂稀释度，Etest和MTS的MIC值与BMD的MIC值没有显着差异，尽管它们倾向于高1至2倍。琼脂稀释的基本一致性为95.6％，Etest为90.6％，MTS为85.2％。琼脂稀释度的类别一致性为98.0％，圆盘扩散的类别一致性为73.1％，Etest的为96.3％，MTS的为96.6％。总之，琼脂稀释和Etest对亚胺培南-雷巴坦产生的结果与BMD相当。