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Hippocampal and lateral entorhinal cortex physiological activity during trace conditioning under urethane anesthesia.
Journal of Neurophysiology ( IF 2.5 ) Pub Date : 2020-07-29 , DOI: 10.1152/jn.00293.2020
Eliezyer Fermino de Oliveira 1, 2 , Clayton Thomas Dickson 2, 3, 4 , Marcelo Bussotti Reyes 1
Affiliation  

Significant evidence shows that the acquisition of delay conditioning can occur in out-of-awareness states, such as under anesthesia. However, it is unclear to what extent and what type of conditioning animals may achieve during non-awake states. Trace conditioning is an appealing protocol to study under anesthesia, given the long empty gap separating the conditioned and unconditioned stimuli, which must be bridged for acquisition to happen. Here, we show evidence that rats display physiological activity during the trace conditioning paradigm under anesthesia. We recorded the activity of the hippocampus (HPC) and lateral entorhinal cortex (LEC) in urethane-anesthetized rats, along with an electromyogram and the electrocardiogram. The protocol consisted of randomly presenting two distinct sound stimuli (CS- and CS+), where only one of them (CS+) was assigned to be trace-paired with a footshock. A trial-average analysis revealed that animals developed a significant climbing heart rate activity initiating at the CS onset and persisting during the trace period. Such climbing arose for both CS- and CS+ with similar slopes, but different intercepts, suggesting CS+ heart rates typically above CS-. The power and coherence of HPC and LEC high frequency bands (> 100 Hz) significantly increased during CS presentation and trace, similarly to both CS- and CS+ and insensitive to either activated or deactivated states. To the best of our knowledge, this is the first attempt to perform a trace conditioning protocol under anesthesia. Confirmation of this procedure acquisition can allow a new preparation for the exploration of brain mechanisms that bind time-discontinuous events.

中文翻译:

氨基甲酸乙酯麻醉下微量调理期间海马和外侧内嗅皮层的生理活动。

重要证据表明,延迟条件反射的获得可能发生在无意识状态下,例如在麻醉状态下。然而,目前尚不清楚动物在非清醒状态下可以达到何种程度和何种类型的条件反射。微量调节是一种在麻醉下进行研究的有吸引力的协议,因为有条件的和无条件的刺激之间有很长的空白间隙,必须桥接才能发生采集。在这里,我们展示了大鼠在麻醉下的微量调节范式期间表现出生理活动的证据。我们记录了氨基甲酸乙酯麻醉大鼠的海马体 (HPC) 和外侧内嗅皮层 (LEC) 的活动,以及肌电图和心电图。该协议包括随机呈现两种不同的声音刺激(CS- 和 CS+),其中只有一个 (CS+) 被指定为与足震配对。试验平均分析显示,动物在 CS 发作时开始出现显着的爬升心率活动,并在跟踪期间持续存在。CS- 和 CS+ 都出现了这种爬升,具有相似的斜率,但截距不同,表明 CS+ 心率通常高于 CS-。HPC 和 LEC 高频段 (> 100 Hz) 的功率和相干性在 CS 呈现和跟踪期间显着增加,类似于 CS- 和 CS+ 并且对激活或停用状态不敏感。据我们所知,这是第一次尝试在麻醉下执行微量调理协议。
更新日期:2020-08-20
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